Transgenic and Targeting Shared Resource

转基因与靶向共享资源 项目总结/摘要 转基因和靶向共享资源（转基因SR）是Case Comprehensive的重要资产 癌症中心（案例CCC）成员的研究，说明了科学计划参与的广度， 仅去年一年，就有45名调查人员，其中40%的人 2005年为Case CCC会员，占总使用量的39%，其中7个项目中有5个使用了 Sr.转基因SR制造转基因、嵌合、敲除和敲入小鼠，并提供以下服务： 再衍生、冷冻保存、复活、体外受精和手术。成功的核心 该设施在咨询、教育和培训方面发挥的作用， 以及售后服务支持。转基因SR的具体目的是： 1.为会员提供咨询、教育和培训。转基因研究所所长 SR咨询癌症中心成员及其研究人员，以确保他们利用最好的 技术，以满足他们的实验目标。CRISPR/Cas9技术的快速发展使得 咨询尤为重要。 2.为Case CCC成员提供新的小鼠遗传模型。转基因SR产生遗传 以及时和具有成本效益的方式， 3.通过直接互动和丰富的 设施网站上的信息。 在上次审查中，转基因SR被评为杰出优点。在本供资周期（2012 - 2016年）， 2016年），转基因SR为133名注册用户提供了服务，其中41人（31%）是案例CCC 成员，代表该中心的7个方案中的5个。 转基因SR在使用CRISPR/Cas9的基因靶向方面取得了巨大成功。自2014年以来，SR 使用这种方法成功地在小鼠中完成了30多个新的基因靶向突变， 这些基因是致病的错义突变。转基因SR是转基因的先锋 应用CRISPR/Cas9技术的核心。在上一个资助期内，转基因SR协助案例CCC 成员在：产生小鼠模型，人类突变导致癌症;确定癌症易感性 基因座;产生用于肿瘤体内成像的小鼠;以及研究与肿瘤相关的基因的基础生物学。 癌该SR是克利夫兰地区唯一的转基因核心，为整个地区的研究人员提供服务 地区因此，它得到了CWRU的大力支持。所有案例CCC成员都可以共同访问此 管理SR。

TRANSGENIC AND TARGETING SHARED RESOURCE PROJECT SUMMARY/ABSTRACT The Transgenic and Targeting Shared Resource (Transgenic SR) is an essential asset to Case Comprehensive Cancer Center (Case CCC) members' research, illustrated by the breadth of scientific program involvement and reflected in the large number of investigators who use this SR. Last year alone, 45 investigators, 40% of whom were Case CCC members, accounting for 39% of total usage, from 5 out of 7 of the Center’s programs used the SR. The Transgenic SR makes transgenic, chimeric, knockout, and knock-in mice and provides services in rederivation, cryopreservation, reanimation, in vitro fertilization and surgery. Central to the success of investigators with new mouse genetic models is the role this facility plays in consultation, education and training, as well as support after service. The Specific Aims of the Transgenic SR are to: 1. Provide Case CCC members with consultation, education and training. The Director of the Transgenic SR consults with Cancer Center members and their research staff to ensure they utilize the best technology to meet their experimental goals. The rapid evolution of CRISPR/Cas9 technologies makes consultation particularly important. 2. Provide Case CCC members with new mouse genetic models. The Transgenic SR generates the genetic variants of mice in a timely and cost-effective manner. 3. Provide Case CCC members with follow-up support through direct interaction and through the wealth of information available on the facility website. At last review, the Transgenic SR was rated Outstanding Merit. Throughout the current funding cycle (2012- 2016), the Transgenic SR provided services to 133 registered users, 41 (31%) of whom are Case CCC members, representing 5 of the Center’s 7 Programs. The Transgenic SR has had great success in gene targeting using CRISPR/Cas9. Since 2014, the SR has successfully completed over 30 new gene-targeted mutations in mice using this approach, the vast majority of which have been disease-causing missense mutations. The Transgenic SR is in the vanguard of transgenic cores in applying CRISPR/Cas9 technology. In the last funding period, the Transgenic SR assisted Case CCC members in: generating mice that model human mutations leading to cancer; identifying cancer susceptibility loci; generating mice for in vivo imaging of tumors; and investigating the basic biology of genes implicated in cancer. This SR is the only transgenic core in the Cleveland area, and it serves investigators throughout the region. As a consequence, it is strongly supported by CWRU. All Case CCC members have access to this jointly managed SR.