TRANSGENIC AND TARGETING

转基因和靶向

• 批准号: 7529376
• 负责人: RONALD A. CONLON
• 金额: $ 6.44万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-06 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7529376
• 关键词: Accounting; Arts; Basic Science; Communities; Comprehensive Cancer Center; Consultations; Core Facility; Cryopreservation; Ensure; Fee-for-Service Plans; Fertilization in Vitro; Gene Targeting; Generations; Investigation; Laboratories; Malignant Neoplasms; Microinjections; Mission; Mus; Operative Surgical Procedures; Services; Transgenic Organisms; animal facility; base; discount; foot; member; mouse model; tissue culture; tool

The Case Transgenic & Targeting Core Facility is the sole service generating genetically modified mice for the Case Comprehensive Cancer Center (Case CCC) and the Cleveland Biomedical community. The facility makes transgenic and gene-targeted mice, in addition to performing rederivations, in vitro fertilization, cryopreservation, and other services. The facility has a staff of four and a director. The service has a laboratory suite of about 1700 square feet adjacent to a state-of-the-art animal facility, and is equipped with four microinjection stations, two tissue culture hoods, and three surgery hoods. The facility operates on a fee-for-service basis, and provides consultation with all services. The facility supports the Case Comprehensive Cancer Center mission through the generation of mouse models of cancer, and tools and materials for investigation of basic science questions. The facility has not received support from the Case CCC previously. Case CCC members accounted for 42% of the use of the facility in 2004 and 2005. Support from the Case CCC would be used to hire an additional staff member to ensure that service backlogs do not occur. In addition, Case CCC members would benefit from discounted fees for services, at less than the academic average.

凯斯转基因和靶向核心设施是为人类生产转基因小鼠的唯一服务 凯斯综合癌症中心 (Case CCC) 和克利夫兰生物医学界。设施 制造转基因和基因靶向小鼠，除了进行再衍生、体外受精、 冷冻保存等服务。该设施有四名员工和一名主管。该服务有一个 实验室套房面积约 1700 平方英尺，毗邻最先进的动物设施，并配备 四个显微注射站、两个组织培养罩和三个手术罩。该设施运行于 按服务收费，并提供所有服务的咨询。该设施支持该案件 综合癌症中心的任务是通过生成小鼠癌症模型以及工具和 用于研究基础科学问题的材料。该设施尚未获得案件的支持 以前的CCC。 2004 年和 2005 年，Case CCC 成员占该设施使用量的 42%。 案例 CCC 的支持将用于雇用一名额外的工作人员，以确保服务 不会出现积压情况。此外，Case CCC 会员还可享受服务费用折扣，价格为 低于学术平均水平。