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Mapping the cell specific DNA damage-induced molecular and bioelectrical responses in the 3D cardiac unit

绘制 3D 心脏单元中细胞特异性 DNA 损伤诱导的分子和生物电反应

基本信息

批准号：
10534185
负责人：
Tzahi Cohen-Karni
金额：
$ 62.32万
依托单位：
CARNEGIE-MELLON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-12-15 至 2026-11-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10534185
关键词：
3-Dimensional Action Potentials Address Age Anthracycline Architecture Biochemical Biological CRISPR/Cas technology Calcium Cancer Survivor Cardiac Cardiac Myocytes Cardiac health Cardiovascular Diseases Cell Aging Cell Communication Cell Culture Techniques Cells Coculture Techniques Crista ampullaris Cultured Cells DNA DNA Damage DNA Markers DNA Repair Endonuclease DNA mapping Data Data Science Deterioration Dilated Cardiomyopathy Disease Dissection ERCC1 gene Electrophysiology (science)Event Exhibits Fibroblasts Frequencies Functional disorder Genes Genotoxic Stress Heart Diseases Heart failure Human Intervention Investigation Ionizing radiation Life Link Lipids Machine Learning Maps Measurement Mediating Membrane Potentials Metabolic Mitochondria Molecular Mus Myocardial dysfunction Myocardium Myofibrils Nature Nuclear Optics Organoids Oxidative Stress Pathogenesis Pathologic Pathology Patients Pattern Pharmaceutical Preparations Prevention strategy Primary idiopathic dilated cardiomyopathy Production Public Health Qualifying Reactive Oxygen Species Role Series Source Specificity Surface System TP53 gene Target Populations Testing Therapeutic Therapeutic Intervention Time Tissues Treatment Protocols Work autocrine bioelectricity biological adaptation to stress cardiac tissue engineering cardiovascular disorder risk cell dimension cell type comparison control design falls genotoxicity heart damage heart dimension/size heart electrical activity heart function imaging modality in vivo induced pluripotent stem cell induced pluripotent stem cell technology inhibitor innovation insight live cell imaging millisecond mitochondrial dysfunction mitochondrial membrane nanofabrication novel novel therapeutic intervention paracrine pharmacologic repaired response senescence sensor spatiotemporal temporal measurement tool treatment strategy two-dimensional

项目摘要

PROJECT SUMMARY This project will test the hypothesis that DNA damage in cardiomyocytes activates p53 leading to mitochondrial alterations and secretion of paracrine factors that drive heart failure. The premise for this has been established from our preliminary data and from the work of others. First, DNA damage and activated DNA damage response (DDR) have been observed in cardiovascular disease (CVD) in humans. Second, studies also show evidence that multiple cell types in the cardiac unit, including cardiomyocytes (CM) and cardiac fibroblasts (CF) display markers of DNA damage and cellular senescence in several disease pathologies. Third, we have recently identified that nuclear DNA damage drives dilated cardiomyopathy. Specifically, cardiomyocyte-depletion of the DNA repair endonuclease, ERCC1-XPF in mice, upregulates the DNA damage response gene, p53, and leads to irregular mitochondrial cristae, accumulation of lipids and increased oxidative stress. Additionally, there is an increase in several cardiac failure and senescence associated markers. However, the exact molecular underpinnings and cell-specificity of these DNA damage-induced changes is poorly understood. One barrier to addressing this question in vivo has been lack of appropriate tools, where DNA damage can be introduced in only one cell type (e.g., CM) and its effect on CF and cardiac function can be investigated. Additionally, 2D cell culture and co-culture systems fall short, as they cannot reproduce tissue dynamics present in a cardiac unit. Herein, we have developed several tools enable the study of cell-cell communication of 3D multicellular system. Specific Aim 1 will map the molecular, functional, and architectural changes upon loss of ERCC1 in CM. In this aim, we will test the mechanistic role of p53 and reactive oxygen species on a number of cellular and mitochondrial parameters, as well as cardiomyocyte electrophysiology. Specific Aim 2 will test whether stochastic, spontaneous DNA damage in the CM or CF drives cardiac electromechanical dysfunction in a cell- autonomous or cell non-autonomous manner through a paracrine effect on neighboring cells. Here, we will analyze the pathological secretome upon genotoxic stress, as well as test the role of eliminating senescent cells on cardiac health. This work is technically innovative as it uses a number of unique tools including concomitant optical and bioelectrical measurements in 3D cardiac organoids. These contributions will be significant because DNA damage is unavoidable and intimately linked to cardiac health and disease. Our team is uniquely qualified to perform this work, with expertise in DNA damage/ repair, cellular senescence, nanofabrication, human iPSC- derived cardiac tissue engineering, and data science. This analysis, we believe, will increase our fundamental understanding of the connection between DNA damage and heart disease and potentially pave the way for new treatment strategies.

项目总结