Lipid Mass Spectrometry

脂质质谱分析

• 批准号: 10543869
• 负责人: Jeffrey G McDonald
• 金额: $ 30.34万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-01-01 至 2026-12-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10543869
• 关键词: Anabolism; Animals; Bile Acids; Catabolism; Cholesterol Esters; Chromatography; Complement; Complex; Coupled; Cultured Cells; Data; Deuterium; Eicosanoids; Ensure; Equipment; Fatty Acids; Genes; Goals; High Pressure Liquid Chromatography; Individual; Isotopes; Leadership; Lipids; Liquid substance; Mass Spectrum Analysis; Mathematics; Measurement; Measures; Metabolic Pathway; Methods; Nitrogen; Noise; Pathway interactions; Phospholipids; Play; Preparation; Program Research Project Grants; Publications; Research; Research Personnel; Research Project Grants; Residual state; Resolution; Robot; Role; Sampling; Services; Signal Transduction; Specificity; Sphingolipids; Stable Isotope Labeling; Steroids; Sterols; Techniques; Time; cardiovascular risk factor; experience; experimental study; improved; insight; instrument; lipid metabolism; lipidomics; liquid chromatography mass spectrometry; mass spectrometer; novel; novel strategies; programs; stable isotope; ultra high resolution

Core 2 – Lipid Mass Spectrometry provides multiple essential services to every investigator involved with this Program Project Grant. These services include lipid analysis measurements as well as novel and unique lipid measurements that provide unparalleled and comprehensive data providing insight into lipid metabolism. The Core is directed by Dr. Jeff McDonald, who has led the lipid mass spectrometry efforts at UT Southwestern for 17 years. Services include mass spectrometry-based quantification of individual lipid species including sterols, oxysterols, sphingolipids, steroids, bile acids, and fatty acids. Qualitative or semi-quantitative analysis of all lipid classes can be measured providing relative comparisons between sample groups and fatty acid compositions for cholesteryl esters, glycerolipids, and other lipid classes. Unique lipid mass spectrometry measurements center around use of ultra-high resolution mass spectrometry to distinctly measure deuterium incorporation into lipids instead of mathematically deconvoluting 2H signals which overlap with the naturally occurring 13C isotope mass envelope. Using this method results in a signal-to-noise ratio that was at best 1 or 2 using traditional mass spectrometry (unit resolution) techniques to a signal-to-noise of 1000 or greater. This requires very advanced mass spectrometers, two of which are currently in our facility. Coupled with advanced chromatography methods that resolve lipids and lipid classes, the synthesis and flow of lipids through a designated biosynthetic or catabolic pathway in cultured cells and living animals is now feasible. The complex and expensive equipment needed to provide these services include multiple mass spectrometers, a liquid handling robot for sample preparation, 96-well SPE and nitrogen blow-down manifolds for sample processing. The lipid mass spectrometry measurements provided by Core 2 will play a major role in every specific aim in this Program Project and in every publication that arises from the completed research.

核心2 -脂质质谱法为每一位参与的研究者提供多种基本服务 这个项目的资助。这些服务包括血脂分析测量以及新的和 独特的血脂测量，提供无与伦比的全面数据，深入了解血脂 新陈代谢.核心是由杰夫麦克唐纳博士，谁领导的脂质质谱工作在UT 西南17年 服务包括基于质谱的单个脂质种类（包括甾醇）的定量， 氧固醇、鞘脂、类固醇、胆汁酸和脂肪酸。所有脂质的定性或半定量分析 提供样品组和脂肪酸组成之间的相对比较 用于胆固醇酯、甘油脂和其它脂类。 独特的脂质质谱测量围绕超高分辨率质谱的使用 光谱法，以清楚地测量氘掺入脂质，而不是数学去卷积 2 H信号，其与天然存在的13 C同位素质量包络重叠。使用此方法会导致 使用传统质谱法（单位分辨率）技术最多为1或2的信噪比与使用常规质谱法（单位分辨率）技术最多为1或2的信噪比相比， 信噪比为1000或更高。这需要非常先进的质谱仪，其中两个目前 在我们的设施。再加上先进的色谱方法，解决脂质和脂质类， 在培养的细胞和活体中，脂质通过指定的生物合成或分解代谢途径的合成和流动 动物是可行的。 提供这些服务所需的复杂且昂贵的设备包括多个质量 光谱仪、用于样品制备的液体处理机器人、96孔SPE和氮气排污歧管 用于样品处理。 核心2提供的脂质质谱测量将在每个特定的研究中发挥重要作用。 在本计划项目和完成的研究所产生的每一个出版物的目的。