Lipid Mass Spectrometry

脂质质谱分析

• 批准号: 10543869
• 负责人: Jeffrey G McDonald
• 金额: $ 30.34万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-01-01 至 2026-12-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10543869
• 关键词: Anabolism; Animals; Bile Acids; Catabolism; Cholesterol Esters; Chromatography; Complement; Complex; Coupled; Cultured Cells; Data; Deuterium; Eicosanoids; Ensure; Equipment; Fatty Acids; Genes; Goals; High Pressure Liquid Chromatography; Individual; Isotopes; Leadership; Lipids; Liquid substance; Mass Spectrum Analysis; Mathematics; Measurement; Measures; Metabolic Pathway; Methods; Nitrogen; Noise; Pathway interactions; Phospholipids; Play; Preparation; Program Research Project Grants; Publications; Research; Research Personnel; Research Project Grants; Residual state; Resolution; Robot; Role; Sampling; Services; Signal Transduction; Specificity; Sphingolipids; Stable Isotope Labeling; Steroids; Sterols; Techniques; Time; cardiovascular risk factor; experience; experimental study; improved; insight; instrument; lipid metabolism; lipidomics; liquid chromatography mass spectrometry; mass spectrometer; novel; novel strategies; programs; stable isotope; ultra high resolution

Core 2 – Lipid Mass Spectrometry provides multiple essential services to every investigator involved with this Program Project Grant. These services include lipid analysis measurements as well as novel and unique lipid measurements that provide unparalleled and comprehensive data providing insight into lipid metabolism. The Core is directed by Dr. Jeff McDonald, who has led the lipid mass spectrometry efforts at UT Southwestern for 17 years. Services include mass spectrometry-based quantification of individual lipid species including sterols, oxysterols, sphingolipids, steroids, bile acids, and fatty acids. Qualitative or semi-quantitative analysis of all lipid classes can be measured providing relative comparisons between sample groups and fatty acid compositions for cholesteryl esters, glycerolipids, and other lipid classes. Unique lipid mass spectrometry measurements center around use of ultra-high resolution mass spectrometry to distinctly measure deuterium incorporation into lipids instead of mathematically deconvoluting 2H signals which overlap with the naturally occurring 13C isotope mass envelope. Using this method results in a signal-to-noise ratio that was at best 1 or 2 using traditional mass spectrometry (unit resolution) techniques to a signal-to-noise of 1000 or greater. This requires very advanced mass spectrometers, two of which are currently in our facility. Coupled with advanced chromatography methods that resolve lipids and lipid classes, the synthesis and flow of lipids through a designated biosynthetic or catabolic pathway in cultured cells and living animals is now feasible. The complex and expensive equipment needed to provide these services include multiple mass spectrometers, a liquid handling robot for sample preparation, 96-well SPE and nitrogen blow-down manifolds for sample processing. The lipid mass spectrometry measurements provided by Core 2 will play a major role in every specific aim in this Program Project and in every publication that arises from the completed research.

核心2 - 脂质质谱法为参与的每个研究者提供了多个基本服务 使用此计划项目赠款。这些服务包括脂质分析测量以及新颖和新颖 独特的脂质测量值，可提供无与伦比的全面数据，可洞悉脂质 代谢。核心由杰夫·麦克唐纳（Jeff McDonald）博士指导，他领导了UT脂质质谱工作 西南17年。 服务包括基于质谱的单个脂质物种的定量，包括立体声， 氧蛋白酶，鞘脂，类固醇，胆汁酸和脂肪酸。所有脂质的定性或半定量分析 可以测量类样品组和脂肪酸组成之间的相对比较 用于胆固醇酯，甘油脂质和其他脂质类。 独特的脂质质谱测量值围绕使用超高分辨率质量 光谱法明显测量肠道氘掺入脂质而不是数学上的卷积 2H信号与天然发生的13C同位素质量包膜重叠。使用此方法导致 信噪比最多使用传统质谱（单位分辨率）技术为1或2 信噪比为1000或更高。这需要非常高级的质谱仪，其中两个是 在我们的设施中。再加上高级色谱方法，可以解决脂质和脂质类， 培养细胞中脂质通过指定的生物合成或分解代谢途径的合成和流动 动物现在可行。 提供这些服务所需的复杂且昂贵的设备包括多个质量 光谱仪，用于样品制备的液体处理机器人，96孔SPE和氮气吹歧管 用于样品处理。 Core 2提供的脂质质谱测量结果将在每个特定的特定方面发挥重要作用 瞄准该计划项目，以及完成的研究。