Lipid Mass Spectrometry

脂质质谱分析

• 批准号: 10543869
• 负责人: Jeffrey G McDonald
• 金额: $ 30.34万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-01-01 至 2026-12-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10543869
• 关键词: Anabolism; Animals; Bile Acids; Catabolism; Cholesterol Esters; Chromatography; Complement; Complex; Coupled; Cultured Cells; Data; Deuterium; Eicosanoids; Ensure; Equipment; Fatty Acids; Genes; Goals; High Pressure Liquid Chromatography; Individual; Isotopes; Leadership; Lipids; Liquid substance; Mass Spectrum Analysis; Mathematics; Measurement; Measures; Metabolic Pathway; Methods; Nitrogen; Noise; Pathway interactions; Phospholipids; Play; Preparation; Program Research Project Grants; Publications; Research; Research Personnel; Research Project Grants; Residual state; Resolution; Robot; Role; Sampling; Services; Signal Transduction; Specificity; Sphingolipids; Stable Isotope Labeling; Steroids; Sterols; Techniques; Time; cardiovascular risk factor; experience; experimental study; improved; insight; instrument; lipid metabolism; lipidomics; liquid chromatography mass spectrometry; mass spectrometer; novel; novel strategies; programs; stable isotope; ultra high resolution

Core 2 – Lipid Mass Spectrometry provides multiple essential services to every investigator involved with this Program Project Grant. These services include lipid analysis measurements as well as novel and unique lipid measurements that provide unparalleled and comprehensive data providing insight into lipid metabolism. The Core is directed by Dr. Jeff McDonald, who has led the lipid mass spectrometry efforts at UT Southwestern for 17 years. Services include mass spectrometry-based quantification of individual lipid species including sterols, oxysterols, sphingolipids, steroids, bile acids, and fatty acids. Qualitative or semi-quantitative analysis of all lipid classes can be measured providing relative comparisons between sample groups and fatty acid compositions for cholesteryl esters, glycerolipids, and other lipid classes. Unique lipid mass spectrometry measurements center around use of ultra-high resolution mass spectrometry to distinctly measure deuterium incorporation into lipids instead of mathematically deconvoluting 2H signals which overlap with the naturally occurring 13C isotope mass envelope. Using this method results in a signal-to-noise ratio that was at best 1 or 2 using traditional mass spectrometry (unit resolution) techniques to a signal-to-noise of 1000 or greater. This requires very advanced mass spectrometers, two of which are currently in our facility. Coupled with advanced chromatography methods that resolve lipids and lipid classes, the synthesis and flow of lipids through a designated biosynthetic or catabolic pathway in cultured cells and living animals is now feasible. The complex and expensive equipment needed to provide these services include multiple mass spectrometers, a liquid handling robot for sample preparation, 96-well SPE and nitrogen blow-down manifolds for sample processing. The lipid mass spectrometry measurements provided by Core 2 will play a major role in every specific aim in this Program Project and in every publication that arises from the completed research.

Core 2-Lipid MS为每一位研究人员提供多种基本服务 有了这项计划项目拨款。这些服务包括血脂分析测量以及新颖和 独特的血脂测量，提供无与伦比的全面数据，深入了解血脂 新陈代谢。核心是由杰夫·麦克唐纳博士执导的，他领导了德克萨斯大学的脂质谱工作 在西南部待了17年。 服务包括基于质谱学的个别类脂物种的量化，包括甾醇， 氧甾醇、神经鞘脂脂、类固醇、胆汁酸和脂肪酸。所有脂类的定性或半定量分析 可以测量类别，提供样本组和脂肪酸组成之间的相对比较 适用于胆固醇酯、甘油脂和其他脂类。 独特的脂质谱测量以超高分辨率质量的使用为中心 用光谱分析方法清楚地测量脂质中的氚掺入量，而不是用数学方法去卷积 与自然产生的13C同位素质量包络重叠的2H信号。使用此方法会导致 使用传统的质谱学(单位分辨率)技术，信噪比至多为1或2 信噪比为1000或更高。这需要非常先进的质谱仪，其中两个目前 在我们的设施里。结合先进的层析方法来分解脂质和类脂类， 脂类在培养细胞和活体中通过指定的生物合成或分解代谢途径的合成和流动 动物现在是可行的。 提供这些服务所需的复杂和昂贵的设备包括多个 光谱仪，用于样品制备的液体处理机器人，96孔固相萃取和氮气排放歧管 用于样品处理。 Core2提供的脂质谱测量将在每一个特定的 在本计划项目和已完成研究产生的每一份出版物中都有目标。