CORE 2 - Mass Spectrometry Core

项目摘要/摘要-核心2-脂质谱核心 脂质谱核心(LMSC)将利用德克萨斯大学的强脂质谱培养 西南部的人类营养中心，已经开发并由杰夫·麦克唐纳博士管理 以满足德克萨斯大学西南部NORC调查人员的需求。通过中心的一套广泛的仪器 除了在基于质谱学的脂肪分析的各个方面都有丰富的经验和知识外， 这个核心的目标是支持所有NORC调查人员提供与他们的 北大西洋公约组织的研究项目。LMSC的三个主要目标是：1)提供定量分析 靶向脂类，包括脂肪酸、甾醇/氧合甾醇、胆汁酸、类固醇、二十烷醇和 鞘脂；2)提供非靶向的相对脂组分析，涵盖主要种类的脂类，包括 酯、甘油脂、磷脂和鞘脂；以及3)开发新的方法来支持独特的 NORC调查人员的需求。LMSC提供的服务和能力几乎涵盖了 从提取到分析再到数据解释和传播的脂类分析。LMSC已经证明 从有效处理大量队列(临床和研究)以进行靶向血脂分析到广泛的能力 小型试点项目的探索性工作。

PROJECT SUMMARY/ABSTRACT – Core 2-Lipid Mass Spectrometry Core The Lipid Mass Spectrometry Core (LMSC) will draw on the strong lipid mass spectrometry culture at UT Southwestern in the Center for Human Nutrition that has been developed and is managed by Dr. Jeff McDonald to serve needs of UT Southwestern NORC investigators. Through an extensive suite of instruments in the Center along with significant experience and knowledge in all aspects of mass spectrometry-based lipid analysis, the goal of this Core is to support all NORC investigators to provide lipid measurements needed relevant to their NORC research projects. The three primary objectives of the LMSC are: 1) Provide quantitative analysis of targeted lipid classes that include fatty acids, sterols/oxysterols, bile acids, steroids, eicosanoids, and sphingolipids; 2) Provide untargeted, relative lipidomic analysis that covers the major classes of lipids including steryl esters, glycerolipids, phospholipids, and sphingolipids; and 3) Develop novel methods to support unique needs of NORC investigators. The services and capabilities provided by the LMSC cover almost all aspects of lipid analysis from extraction to analysis to data interpretation and dissemination. The LMSC has proven capabilities from effectively handling large cohorts (clinical and research) for targeted lipid analysis to broad exploratory work on small pilot projects.