CORE 2 - Mass Spectrometry Core

CORE 2 - 质谱核心

• 批准号: 10512734
• 负责人: Jeffrey G McDonald
• 金额: $ 16.4万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-01 至 2027-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10512734
• 关键词: Abbreviations; Bile Acids; Clinical Research; Communities; Consultations; Contract Services; Contracts; Data Analyses; Education; Eicosanoids; Ensure; Esters; Experimental Designs; Fatty Acids; Funding; Goals; Grant; Human; Knowledge; Laboratories; Lipid Biochemistry; Lipids; Maintenance; Mass Fragmentography; Mass Spectrum Analysis; Measurement; Medical center; Methods; Obesity; Phospholipids; Physiology; Pilot Projects; Quality Control; Research; Research Personnel; Research Project Grants; Sampling; Services; Sphingolipids; Steroids; Sterols; Training; Work; base; cohort; data dissemination; experience; innovation; instrument; lipidomics; liquid chromatography mass spectrometry; novel; nutrition; operation

PROJECT SUMMARY/ABSTRACT – Core 2-Lipid Mass Spectrometry Core The Lipid Mass Spectrometry Core (LMSC) will draw on the strong lipid mass spectrometry culture at UT Southwestern in the Center for Human Nutrition that has been developed and is managed by Dr. Jeff McDonald to serve needs of UT Southwestern NORC investigators. Through an extensive suite of instruments in the Center along with significant experience and knowledge in all aspects of mass spectrometry-based lipid analysis, the goal of this Core is to support all NORC investigators to provide lipid measurements needed relevant to their NORC research projects. The three primary objectives of the LMSC are: 1) Provide quantitative analysis of targeted lipid classes that include fatty acids, sterols/oxysterols, bile acids, steroids, eicosanoids, and sphingolipids; 2) Provide untargeted, relative lipidomic analysis that covers the major classes of lipids including steryl esters, glycerolipids, phospholipids, and sphingolipids; and 3) Develop novel methods to support unique needs of NORC investigators. The services and capabilities provided by the LMSC cover almost all aspects of lipid analysis from extraction to analysis to data interpretation and dissemination. The LMSC has proven capabilities from effectively handling large cohorts (clinical and research) for targeted lipid analysis to broad exploratory work on small pilot projects.

项目总结/摘要-核心2-脂质质谱核心 脂质质谱核心（LMSC）将借鉴UT强大的脂质质谱文化 西南部的人类营养中心，已开发和管理的杰夫麦克唐纳博士 以满足UT西南NORC调查人员的需求。通过该中心的一系列仪器， 沿着在基于质谱的脂质分析的各个方面的丰富经验和知识， 该核心的目标是支持所有NORC研究者提供与他们的研究相关的所需血脂测量结果。 NORC研究项目。LMSC的三个主要目标是：1）提供定量分析， 靶向脂质类，包括脂肪酸、固醇/氧化固醇、胆汁酸、类固醇、类二十烷酸，以及 鞘脂; 2）提供非靶向的相对脂质组学分析，涵盖主要类别的脂质，包括 甾醇酯、甘油脂、磷脂和鞘脂;以及3）开发新的方法来支持独特的 NORC调查员的需求。LMSC提供的服务和能力几乎涵盖了 脂质分析从提取到分析再到数据解释和传播。LMSC已经证明 从有效处理大型队列（临床和研究）进行靶向血脂分析， 开展小型试点项目的探索性工作。