CORE 2 - Mass Spectrometry Core

CORE 2 - 质谱核心

• 批准号: 10512734
• 负责人: Jeffrey G McDonald
• 金额: $ 16.4万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-01 至 2027-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10512734
• 关键词: Abbreviations; Bile Acids; Clinical Research; Communities; Consultations; Contract Services; Contracts; Data Analyses; Education; Eicosanoids; Ensure; Esters; Experimental Designs; Fatty Acids; Funding; Goals; Grant; Human; Knowledge; Laboratories; Lipid Biochemistry; Lipids; Maintenance; Mass Fragmentography; Mass Spectrum Analysis; Measurement; Medical center; Methods; Obesity; Phospholipids; Physiology; Pilot Projects; Quality Control; Research; Research Personnel; Research Project Grants; Sampling; Services; Sphingolipids; Steroids; Sterols; Training; Work; base; cohort; data dissemination; experience; innovation; instrument; lipidomics; liquid chromatography mass spectrometry; novel; nutrition; operation

PROJECT SUMMARY/ABSTRACT – Core 2-Lipid Mass Spectrometry Core The Lipid Mass Spectrometry Core (LMSC) will draw on the strong lipid mass spectrometry culture at UT Southwestern in the Center for Human Nutrition that has been developed and is managed by Dr. Jeff McDonald to serve needs of UT Southwestern NORC investigators. Through an extensive suite of instruments in the Center along with significant experience and knowledge in all aspects of mass spectrometry-based lipid analysis, the goal of this Core is to support all NORC investigators to provide lipid measurements needed relevant to their NORC research projects. The three primary objectives of the LMSC are: 1) Provide quantitative analysis of targeted lipid classes that include fatty acids, sterols/oxysterols, bile acids, steroids, eicosanoids, and sphingolipids; 2) Provide untargeted, relative lipidomic analysis that covers the major classes of lipids including steryl esters, glycerolipids, phospholipids, and sphingolipids; and 3) Develop novel methods to support unique needs of NORC investigators. The services and capabilities provided by the LMSC cover almost all aspects of lipid analysis from extraction to analysis to data interpretation and dissemination. The LMSC has proven capabilities from effectively handling large cohorts (clinical and research) for targeted lipid analysis to broad exploratory work on small pilot projects.

项目摘要/摘要 - 核心2-lipid质谱核心核心 脂质质谱核心（LMSC）将在UT的强脂质质谱培养 已经开发的人类营养中心的西南部，由杰夫·麦克唐纳博士管理 满足西南诺克调查人员的需求。通过中心的大量乐器 以及基于质谱分析的各个方面的重要经验和知识， 该核心的目标是支持所有NORC调查人员提供与其相关的脂质测量 NORC研究项目。 LMSC的三个主要对象是：1）提供定量分析 有针对性的脂质类别，包括脂肪酸，固醇/氧蛋白酶，胆汁酸，类固醇，eicosanoids和 鞘脂； 2）提供未靶向的相对脂肪组分析，涵盖了包括的主要脂质类别 Steryl酯，甘油，磷脂和鞘脂； 3）开发新的方法来支持独特 NORC调查人员的需求。 LMSC提供的服务和功能几乎涵盖了 从提取到分析到数据解释和传播的脂质分析。 LMSC已证明 有效处理大型队列的能力（临床和研究）进行靶向脂质分析到广泛 小型试点项目的探索性工作。