Neuronal HDAC9, Synaptic Plasticity and Alzheimer's Disease

神经元 HDAC9、突触可塑性和阿尔茨海默病

• 批准号: 10554326
• 负责人: Xin-Yun Lu
• 金额: $ 56.76万
• 依托单位: AUGUSTA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-02-01 至 2027-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10554326
• 关键词: APP-PS1; Acceleration; Accounting; Age; Aging; Alzheimer' s Disease; Alzheimer' s disease model; Alzheimer' s disease pathology; Alzheimer' s disease patient; Alzheimer' s disease risk; Amyloid beta-Protein; Amyloid deposition; Automobile Driving; Binding; Brain; Brain region; Brain-Derived Neurotrophic Factor; Cells; Cerebral cortex; Cognition; Cognitive; Cognitive deficits; Complex; Data; Dementia; Development; Disease Progression; Down-Regulation; EZH2 gene; Epigenetic Process; Exhibits; Functional disorder; Future; Genes; Glutamates; HDAC9 gene; Hippocampus; Histone Deacetylase; Impaired cognition; Impairment; Knockout Mice; Learning; Link; Mediating; Mediator; Memory; Memory Loss; Memory impairment; Modification; Molecular; Mus; Nerve Degeneration; Nerve Growth Factors; Neurodegenerative Disorders; Neurons; Neuropeptides; Neurotrophin 3; Pathology; Peripheral; Play; Polycomb; Prefrontal Cortex; Process; Protein Isoforms; Reporting; Role; Synapses; Synaptic plasticity; Testing; Therapeutic Intervention; Tissues; Wild Type Mouse; Zinc; age effect; age related neurodegeneration; aging brain; base; candidate identification; cognitive enhancement; cognitive function; histone methyltransferase; improved; insight; mouse model; myocyte-specific enhancer-binding-factor 2C; neuronal pentraxin; neuropathology; neurotransmission; overexpression; pathological aging; pharmacologic; pre-clinical; promoter; recruit; tau Proteins; transcription factor; transcriptome sequencing

PROJECT SUMMARY Alzheimer’s disease (AD) is an age-related neurodegenerative disorder that causes memory loss and cognitive decline. Synaptic dysfunction and loss correlates strongly with cognitive impairment in AD. Aging is the leading risk factor for AD, and epigenetic mechanisms involving histone deacetylases (HDACs) play an important role in aging and age-related neurodegenerative disorders. Among the 11 zinc-dependent HDACs, HDAC9 is the most abundant isoform in the brain, found exclusively in neurons. We provide key preliminary data showing that HDAC9 expression in the hippocampus and prefrontal cortex (PFC) diminishes with aging in wild-type mice, and that reduced HDAC9 expression precedes the onset of amyloid deposition in the APP/PS1 mouse model of AD. Consistent with these preclinical findings, AD patients exhibited decreased HDAC9 expression in the dorsolateral PFC. Moreover, global or hippocampal CA1-specific deletion of HDAC9 induces cognitive impairment and impairs synaptic plasticity, while HDAC9 overexpression produces cognitive-enhancing effects. We hypothesize that reduced neuronal HDAC9 mediates cognitive decline, synaptic dysfunction and other neuropathologies associated with brain aging and AD. To test this hypothesis, we propose three specific aims. In Aim 1, we will test the hypothesis that loss of HDAC9 in hippocampal and PFC neurons mediates age- and AD-related neuropathology and cognitive impairment. In Aim 2, we will test the hypothesis that the histone methyltransferase EZH2 [the catalytic component of the polycomb repressive complex 2 (PRC2), which catalyzes repressive H3K27me3 modifications at gene promoters] epigenetically silences HDAC9 expression in the hippocampus and PFC during aging and in AD. In Aim 3, we will test the hypothesis that neuronal pentraxin 2 (NP2), nerve growth factor inducible (VGF), and brain-derived neurotrophic factor (BDNF) mediate the downstream effects of HDAC9 on hippocampal synaptic plasticity and cognition. We expect that the results will provide insight into molecular mechanisms underlying the epigenetic control of genes related to aging and AD and offer potential targets for future therapeutic interventions.

项目总结