Irradiation, Preclinical Imaging, & Microscopy (IPIM)

辐照、临床前成像、

• 批准号: 10554257
• 负责人: Jack Hoopes
• 金额: $ 15.75万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-04 至 2024-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10554257
• 关键词: Angiography; Animals; Bioluminescence; Biotechnology; Brain Neoplasms; Budgets; Cancer Center; Cancer Center Support Grant; Cells; Clinical Research; Communities; Complex; Data Analyses; Dedications; Development; Diagnosis; Diagnostic Imaging; Electron Microscopy; Environment; Equipment; Experimental Designs; Family suidae; Fluorescence Microscopy; Fluoroscopy; Frequencies; Functional Magnetic Resonance Imaging; Funding; Genetic; Head and Neck Cancer; Histopathology; Human; Image; Image Analysis; Imaging Techniques; Inflammation; Institution; Magnetic Resonance Imaging; Maintenance; Malignant Neoplasms; Membrane Protein Traffic; Methods; Microscopy; Microvascular Permeability; Modality; Modeling; Molecular; Molecular Genetics; Molecular Target; NCI Center for Cancer Research; Nerve; Norris Cotton Cancer Center; Operative Surgical Procedures; Optics; Pharmacologic Substance; Positron-Emission Tomography; Productivity; Qualifying; Radiation Oncology; Research; Research Peer Review; Research Personnel; Research Project Grants; Resource Sharing; Resources; Rodent; Scanning Electron Microscopy; Services; Techniques; Technology; Therapeutic; Time; Tissues; Transmission Electron Microscopy; Update; Whole Organism; Workplace; Xenograft procedure; anatomic imaging; angiogenesis; animal imaging; anticancer research; biomedical imaging; cancer surgery; cancer therapy; cellular imaging; clinical translation; cost effective; dosimetry; driving force; experience; image visualization; imager; imaging agent; imaging modality; improved; innovation; instrumentation; irradiation; live cell imaging; member; microscopic imaging; multimodality; new technology; next generation; novel; pre-clinical; preclinical imaging; programs; radiological imaging; tool; tumor; ultrasound

Project Summary/Abstract: Irradiation, Preclinical Imaging, and Microscopy The Irradiation, Preclinical Imaging, and Microscopy Shared Resource (IPIM) provides a comprehensive array of sophisticated cancer relevant diagnostic imaging and therapeutic instrumentation/techniques for cells, tissues, and large and small animals, with a focus on preclinical work that sets the stage for subsequent rapid clinical translation. Irradiation services are provided with a 137Cs irradiator for use on cells and animals. Optical cellular imaging and electron microscopy provides multiple types of confocal, TIRF and wide-field fluorescence microscopy imaging, as well as scanning and transmission electron microscopy. Rodent imaging components include PET, CT, MRI, Bioluminescence/Fluorescent (Xenogen/IVIS), and VisualSonics Vevo 770 high- frequency ultrasound. Large animal imaging includes MRI, fMRI, CT, PET, ultrasound, fluoroscopy/ angiography, and intra-operative MRI/CT as part of the unique Center for Surgical Innovation (CSI). Since its opening during the current funding period, CSI has enabled a total of 7 peer-reviewed research grants, 5 of which are from NCI. Notable CSI projects include development, imaging, and treatment of a human-porcine xenograft brain tumor model, development of MRI-compatible instrumentation for facilitation of head and neck cancer surgeries performed in the MRI-intraoperative environment, and development of a novel intraoperative nerve imaging agent. The capabilities of the CSI are currently available in only a few of the largest clinical research institutions, and IPIM is distinct in having similar capabilities for use in large animal studies. Since 2015, IPIM facilities have been utilized by 55 NCCC Members from all 4 NCCC Research Programs (CPS [2], CBT [22], ICI [16], TEC [15]). NCCC Funded Member Users represented 72% of Total Users, and we request only 20% of Total IPIM budget from CCSG funding. Examples of NCCC Member-supported projects include dual modality/dual agent imaging (Davis, Pogue and Samkoe [TEC]), fundamental mechanisms associated with membrane traffic control microvascular permeability, angiogenesis and inflammation that drive tumor vasculature and subsequent treatment (Stan, Turk, Fiering, Noelle [ICI] and Pogue [TEC]); and Cherenkov irradiation as a real-time dosimetry tool in the radiation oncology setting (Pogue, Gladstone, Hoopes, and Jarvis [TEC]). In addition to providing outstanding viable imaging, IPIM will focus on accurately combining / co- registering the extensive array of radiological imaging techniques with live cell imaging, molecular targeting and genetics that will lead the way into the next generation of diagnosis and therapy. The resource is staffed by highly qualified research directors and experienced resource managers that maintain instrumentation, provide technical support, educate NCCC researchers, and are instrumental in upgrading IPIM technologies. Recent enhancements have included upgrading of the NCCC small animal 9.4T MRI. Pending improvements include replacement of our small animal CT and whole animal IVIS fluorescent/bioluminescent imagers and a dedicated effort to co-register anatomic imaging parameters with molecular histopathology and genetics.

项目总结/摘要：辐照、临床前成像和显微镜检查 辐照、临床前成像和显微镜共享资源（IPIM）提供了一个全面的阵列 先进的癌症相关诊断成像和治疗仪器/技术的细胞， 组织，大型和小型动物，重点是临床前工作，为随后的快速 临床翻译辐照服务配备了用于细胞和动物的137 Cs辐照器。光学 细胞成像和电子显微镜提供了多种类型的共聚焦，TIRF和宽场荧光 显微成像，以及扫描和透射电子显微镜。啮齿动物成像组件 包括PET、CT、MRI、生物发光/荧光（Xenogen/IVIS）和VisualSonics Vevo 770高- 超声波频率大型动物成像包括MRI、fMRI、CT、PET、超声、荧光透视/ 血管造影和术中MRI/CT作为独特的外科创新中心（CSI）的一部分。以来 在目前的资助期内开放，CSI已经实现了7个同行评审的研究赠款， 来自国家癌症研究所值得注意的CSI项目包括开发、成像和治疗人-猪 异种移植脑肿瘤模型，MRI兼容器械的开发，用于促进头颈部 在MRI术中环境中进行的癌症手术，以及开发一种新的术中 神经显像剂CSI的功能目前仅在几个最大的临床 研究机构，和IPIM是不同的，具有类似的能力，用于大型动物研究。以来 2015年，IPIM设施已被所有4个NCCC研究项目（CPS [2]， CBT [22]、ICI [16]、TEC [15]）。NCCC资助的会员用户占总用户的72%，我们要求 只有20%的IPIM总预算来自CCSG资金。NCCC制造商支持的项目示例包括 双模态/双试剂成像（Davis、Pogue和Samkoe [TEC]），相关的基本机制 通过膜交通控制微血管通透性、血管生成和炎症， 血管系统和后续治疗（Stan、Turk、Fiering、Noelle [ICI]和Pogue [TEC]）;和Cherenkov 放射作为放射肿瘤学环境中的实时剂量测定工具（Pogue，Gladstone，Hoopes，和 Jarvis [TEC]）。除了提供出色的可行成像外，IPIM还将专注于准确地结合/协同 将广泛的放射成像技术与活细胞成像、分子靶向 和遗传学，这将引领下一代的诊断和治疗。该资源配备了人员 由高素质的研究总监和经验丰富的资源经理维护仪器， 提供技术支持，教育NCCC研究人员，并在升级IPIM技术的工具。 最近的改进包括升级NCCC小动物9.4T MRI。有待改进 包括更换我们小动物CT和整个动物IVIS荧光/生物发光成像仪， 致力于将解剖成像参数与分子组织病理学和遗传学进行配准。