Biorepository Core B

生物储存库核心B项目总结 博士Claudio Fiocchi是核心总监。Florian里德尔博士担任副主任，Jean-Paul博士 Achkar、Jennifer Ko和Amitabh Chak担任技术总监。 生物储存库核心的目标：具体目标包括：1）获取、处理和分配组织， 来自手术标本和活组织检查的细胞，以及来自胃肠道疾病患者的血清、DNA和粪便， 炎症、癌症和对照用于IRB批准的DDRCC成员项目; 2）提供表型， 生物样品的基因型、临床和人口统计学信息; 3）提供分离、纯化、培养和 使用大多数类型的胃肠道细胞; 4）建议，教导和监督研究人员的原则， 方法，分析和实验设计; 5）监控核心服务，并不断评估 DDRCC成员最大限度地受益于他们的研究优先事项，以及6）在 最具成本效益和最耗时的方式。 生物储藏核心的好处：在资助的前4年， 克利夫兰DDRCC生物储存核心的基本好处是， DDRCC社区未能收到生物标本的类型，大小或数量，以及培训和 他/她的学习所需的后勤支持。这一成功是通过认真制定具体的 目标.核心的一个新的好处是一个国家的最先进的实验室信息系统（LabVantage解决方案-LV）， 其跟踪样本的来源、临床信息、样本的谱系和样本检索， 分布式，并可连接EPIC、Copath、Sunquest等。这一新系统极大地有利于Core用户 通过确保样本质量，快速识别，诊断和交付，以及正式访问临床数据， 实验结果的解释。最后，该中心大大扩大了其技术服务， 包括1）肠类器官产生，2）类器官衍生的上皮细胞单层，3）分离 肠系膜脂肪细胞，4）单细胞分析（Fluidigm C1和10 X Genomics平台），5）CyTOF和 光谱流式细胞术系统，和6）进入食管癌和结肠癌样本的大型生物库。 总体而言，核心通过提供独特和高质量的生物样品和服务使DDRCC成员受益。

BIOREPOSITORY CORE B PROJECT SUMMARY Dr. Claudio Fiocchi is Core Director. Dr. Florian Rieder serves as Associate Director, and Drs. Jean-Paul Achkar, Jennifer Ko and Amitabh Chak serve as Technical Directors. Goals of the Biorepository Core: The specific goals include: 1) Procure, process, and distribute tissue and cells from surgical specimens and biopsies, as well as serum, DNA and stools, from patients with gastrointestinal inflammation, cancer and controls for use in IRB-approved projects of DDRCC members; 2) Provide phenotypic, genotypic, clinical and demographic information of the biosamples; 3) Provide isolation, purification, culture and use of most types of gastrointestinal tract cells; 4) Advise, teach, and supervise investigators on principles, methods, analysis and experimental design; 5) Monitor Core services and continuously evaluate the needs of the DDRCC members to maximally benefit their research priorities, and 6) provide all above services in the most cost-effective and least time-consuming manner. Benefits of the Biorepository Core: During the first 4 years of funding, the objective evidence for the essential benefits of the Cleveland DDRCC Biorepository Core is the fact not a single investigator of the large DDRCC community has failed to receive the type, size or number of biospecimens, as well as the training and logistic support required for his/her studies. This success was achieved by the careful enactment of the specific goals. A new benefit of the Core is a state-of-the-art laboratory information system (LabVantage Solutions-LV), which tracks origin of samples, clinical information, genealogy of samples, and sample retrieval and distribution, and has connectivity to EPIC, Copath, Sunquest, etc. This new system greatly benefits Core users by assuring specimen quality, quick identification, diagnosis and delivery, and formal access to clinical data for interpretation of the experimental results. Finally, the Core has considerably expanded its technical services to include 1) generation of intestinal organoids, 2) organoid-derived epithelial cell monolayers, 3) isolation of mesenteric adipocytes, 4) single cell analysis (Fluidigm C1 and 10X Genomics platforms), 5) CyTOF and spectral flow cytometry systems, and 6) access to a large biobank of esophageal and colon cancer specimens. Overall, the Core benefits DDRCC members by providing unique and high quality biosamples and services.