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Biorepository Core B

生物样本库核心B

基本信息

批准号：
10361544
负责人：
CLAUDIO FIOCCHI
金额：
$ 22.01万
依托单位：
CASE WESTERN RESERVE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-02-15 至 2025-01-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10361544
关键词：
Adipocytes Area Basic Science Biology Biopsy Cancer Control Cell Separation Cells Chronic Classification Clinic Clinical Clinical Data Clinical Investigator Clinical Laboratory Information Systems Clinical Research Colon Carcinoma Constipation Consultations Consumption Crohn&apos s disease DNA Data Analyses Diagnosis Digestive System Disorders Diverticulosis Elements Epidemiology Epithelial Cells Esophagus Experimental Designs Familial Adenomatous Polyposis Syndrome Feces Flow Cytometry Foundations Funding Gastrointestinal tract structure Genealogy Generations Genetic Genotype Goals Human Human Resources In Vitro Individual Inflammation Institution Institutional Review Boards Intestinal Volvulus Intestines Large Intestine Liver diseases Logistics Malignant Neoplasms Malignant neoplasm of esophagus Mesentery Metabolism Methods Monitor Operative Surgical Procedures Organoids Patients Performance Phenotype Physicians Process Research Research Activity Research Institute Research Personnel Research Priority Research Project Grants Resources Retrieval Running Sampling Scientist Serum Services Small Intestines Specimen Supervision System Time Tissues Training Translating Translational Research Ulcerative Colitis Universities bench to bedside biobank cell type community center cost effective cost effectiveness design experience gastrointestinal genomic platform improved member monolayer patient oriented research research study single cell analysis success synergism translational study tumorigenesis

项目摘要

BIOREPOSITORY CORE B PROJECT SUMMARY Dr. Claudio Fiocchi is Core Director. Dr. Florian Rieder serves as Associate Director, and Drs. Jean-Paul Achkar, Jennifer Ko and Amitabh Chak serve as Technical Directors. Goals of the Biorepository Core: The specific goals include: 1) Procure, process, and distribute tissue and cells from surgical specimens and biopsies, as well as serum, DNA and stools, from patients with gastrointestinal inflammation, cancer and controls for use in IRB-approved projects of DDRCC members; 2) Provide phenotypic, genotypic, clinical and demographic information of the biosamples; 3) Provide isolation, purification, culture and use of most types of gastrointestinal tract cells; 4) Advise, teach, and supervise investigators on principles, methods, analysis and experimental design; 5) Monitor Core services and continuously evaluate the needs of the DDRCC members to maximally benefit their research priorities, and 6) provide all above services in the most cost-effective and least time-consuming manner. Benefits of the Biorepository Core: During the first 4 years of funding, the objective evidence for the essential benefits of the Cleveland DDRCC Biorepository Core is the fact not a single investigator of the large DDRCC community has failed to receive the type, size or number of biospecimens, as well as the training and logistic support required for his/her studies. This success was achieved by the careful enactment of the specific goals. A new benefit of the Core is a state-of-the-art laboratory information system (LabVantage Solutions-LV), which tracks origin of samples, clinical information, genealogy of samples, and sample retrieval and distribution, and has connectivity to EPIC, Copath, Sunquest, etc. This new system greatly benefits Core users by assuring specimen quality, quick identification, diagnosis and delivery, and formal access to clinical data for interpretation of the experimental results. Finally, the Core has considerably expanded its technical services to include 1) generation of intestinal organoids, 2) organoid-derived epithelial cell monolayers, 3) isolation of mesenteric adipocytes, 4) single cell analysis (Fluidigm C1 and 10X Genomics platforms), 5) CyTOF and spectral flow cytometry systems, and 6) access to a large biobank of esophageal and colon cancer specimens. Overall, the Core benefits DDRCC members by providing unique and high quality biosamples and services.

生物储存库核心b项目总结