A Facility to Generate Connectomics Information

该方案的目标是传播高分辨率大体积连续切片电子 显微镜数据给神经科学家。利用我们的电子显微镜设备，我们将为用户提供 培训如何使用这些新技术，并提供使用我们专门设施的机会， 他们可以生成永久的数据切片，并创建适合神经网络的数据集， 电路分析或神经和神经胶质细胞类型分析。我们将对我们的 软件，以提高用户数据生产的规模和效率，以满足 用户社区对这些方法缺乏经验。该项目解决了引人注目的 致力于脑2025的第一个高优先级目标的神经科学研究人员的需求 报告：“映射电路的结构和组件”，为他们提供访问工具 否则以其目前的形式是不可用或不切实际的。

The goal of this proposal is to disseminate high resolution large volume serial section electron microscopy data to neuroscientists. Using our electron microscopy facility, we will provide user training on the use of these new technologies and provide access to our specialized facilities so that they may generate permanent ultrathin sections and create data sets amenable for neural circuit analysis or neural and glial cell type analysis. We will make minor improvements in our software to increase the scale and efficiency of user data production to meet the needs of a user community that are inexperienced in these approaches. This project addresses compelling needs of neuroscience researchers who work on the first high priority goal of the BRAIN 2025 report: “Mapping the Structure and Components of Circuits” by providing them access to tools that are otherwise unavailable or impractical in their current form.