A Facility to Generate Connectomics Information

生成连接组学信息的工具

• 批准号: 10596124
• 负责人: Jeff W Lichtman
• 金额: $ 126.22万
• 依托单位: HARVARD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-30 至 2025-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10596124
• 关键词: Address; Brain; Brain Diseases; Cells; Classification; Communities; Computer Assisted; Computer software; Data; Data Set; Devices; Educational workshop; Electron Microscope; Electron Microscopy; Electron Microscopy Facility; Electronic Mail; Environment; Esthetics; Fees; Funding; Goals; Heavy Metals; Human Resources; Image; Intuition; Journals; Laboratories; Learning; Maps; Methods; Microtome - medical device; Minor; Nervous System; Nervous System Physiology; Neuroglia; Neurons; Neurosciences; Organoids; Persons; Plant Resins; Positioning Attribute; Process; Production; Property; Publications; Reporting; Research Personnel; Resolution; Resources; Roentgen Rays; Scanning; Scheme; Services; Silicon; Site Visit; Speed; Stains; Structure; Synapses; Techniques; Technology; Thinness; Tissue Sample; Tissues; Training; United States National Institutes of Health; Voice; Work; Writing; X-Ray Computed Tomography; brain tissue; cell type; improved; instrument; interest; large datasets; nanoscale; neural circuit; neuronal circuitry; new technology; outreach; sample fixation; symposium; tissue preparation; tool

The goal of this proposal is to disseminate high resolution large volume serial section electron microscopy data to neuroscientists. Using our electron microscopy facility, we will provide user training on the use of these new technologies and provide access to our specialized facilities so that they may generate permanent ultrathin sections and create data sets amenable for neural circuit analysis or neural and glial cell type analysis. We will make minor improvements in our software to increase the scale and efficiency of user data production to meet the needs of a user community that are inexperienced in these approaches. This project addresses compelling needs of neuroscience researchers who work on the first high priority goal of the BRAIN 2025 report: “Mapping the Structure and Components of Circuits” by providing them access to tools that are otherwise unavailable or impractical in their current form.

该方案的目标是传播高分辨率的大体积串联截面电子 把显微镜数据传给神经学家。使用我们的电子显微镜设备，我们将为用户提供 提供有关使用这些新技术的培训，并提供使用我们的专门设施的机会 他们可能会产生永久的超薄切片，并创建适合神经的数据集 电路分析或神经和神经胶质细胞类型分析。我们将对我们的产品做一些小改进 提高用户数据生产规模和效率的软件，以满足 在这些方法方面缺乏经验的用户社区。这个项目解决了令人信服的 致力于大脑2025第一个高优先级目标的神经科学研究人员的需求 报告：通过让他们访问工具来绘制电路的结构和元件图 在目前的形式下是无法获得或不切实际的。

项目成果

Volume Electron Microscopy Workflows for the study of Large-Scale Neural Connectomics

用于研究大规模神经连接组学的体积电子显微镜工作流程

• DOI: 10.1093/micmic/ozad067.622
• 发表时间: 2023
• 期刊: Microscopy and Microanalysis
• 影响因子: 2.8
• 作者: Schalek, R L;Petkova, M;Boulanger-Weill, J;Karlupia, N;Wang, S;Wang, X;Dhanyasi, N;Berger, D
• 通讯作者: Berger, D