Zooming into the fish's brain-What is really going on! Connectomics analysis of larval zebrafish.

放大鱼的大脑——到底发生了什么！

• 批准号: 10525435
• 负责人: Jeff W Lichtman
• 金额: $ 97.36万
• 依托单位: HARVARD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-25 至 2027-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10525435
• 关键词: Animals; Atlases; Autonomic Pathways; Autonomic nervous system; Behavior; Behavior Therapy; Behavioral; Blood Vessels; Brain; Cardiac; Cardiovascular system; Cells; Computer Assisted; Data; Desire for food; Electron Microscopy; Environment; Event; Fishes; Futility; Generations; Gills; Goals; Hand; Heart; Image; Infrastructure; Limb structure; Link; Location; Machine Learning; Manuals; Maps; Methods; Mind; Modeling; Motor; Motor Pathways; Muscle; Nervous system structure; Neural Pathways; Neurons; Organ; Output; Oxygen; Pathway interactions; Peripheral; Peripheral Nervous System; Publishing; Reflex action; Regulation; Research; Research Personnel; Resolution; Role; Sensory; Synapses; System; Testing; Time; Validation; Vascular blood supply; Vertebrates; Zebrafish; automated segmentation; base; cell type; computer science; connectome; connectome data; experimental study; high resolution imaging; interest; light microscopy; mind body interaction; neural circuit; reconstruction; relating to nervous system; skeletal; skills; tool

Project 3 The principal goal of Project 3 is to better link the behaviors of zebrafish to underlying synaptic circuitry. In both the central and peripheral nervous systems we will leverage recent advances in high throughput serial section electron microscopy to acquire high resolution image data and then use new machine learning approaches to analyze this data. This effort will use three kinds of approaches: 1) sparse manual reconstructions of particular pathways related to particular behaviors, 2) dense reconstruction of synaptic connections to reveal all the input and output of neurons that is spatially correlated to markers that show excitatory and inhibitory cell types and activity, and 3) saturated reconstructions that also provide glial, vascular, sensory end organs and muscle (cardiac, skeletal and smooth). The zebrafish is the only vertebrate animal where obtaining a saturated reconstruction of the entire nervous system is conceivable. The connectomic data will be used initially to test the validity of published models of how a behavior is instantiated in the nervous system. In the likely event that there is some difference between what was expected based on published models and what is found, these data will be used to help formulate new models. Second, the data will be used to reveal previously unknown pathways, such as those that interconnect the autonomic and somatic nervous systems. Finally we will build the necessary infrastructure so the data can be used as a public, freely addressable atlas for any researcher who has interest in tracking the long and short range neural pathways that underlie vertebrate behavior at the resolution of single synapses.

项目3 项目3的主要目标是更好地将斑马鱼的行为与潜在的突触回路联系起来。两者中 中枢和外周神经系统，我们将利用高通量连续切片电子显微镜的最新进展， 显微镜来获取高分辨率的图像数据，然后使用新的机器学习方法来分析这些数据。这 这项工作将使用三种方法：1）稀疏手动重建特定的路径相关的特定 行为，2）突触连接的密集重建，以揭示空间上神经元的所有输入和输出， 与显示兴奋性和抑制性细胞类型和活性的标志物相关，以及3）饱和重建， 提供神经胶质、血管、感觉终末器官和肌肉（心脏、骨骼和平滑肌）。 斑马鱼是唯一的脊椎动物，其中获得整个神经系统的饱和重建是 可以想象。连接组学数据最初将用于测试已发表的行为如何影响的模型的有效性。 在神经系统中的实例化。在很可能的情况下，基于 公布的模型和发现的数据，这些数据将用于帮助制定新的模型。第二，数据将被使用 揭示以前未知的通路，例如那些连接自主神经系统和躯体神经系统的通路。 最后，我们将建立必要的基础设施，使数据可以作为一个公共的，可自由寻址的地图集， 研究人员谁有兴趣在跟踪长期和短期的神经通路，脊椎动物的行为基础在 单个突触的分辨率。