Brain-gut-retina axis in diabetic retinopathy

糖尿病视网膜病变中的脑-肠-视网膜轴

• 批准号: 10595142
• 负责人: CHARLES J FRAZIER
• 金额: $ 55.06万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-03-01 至 2027-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10595142
• 关键词: Ablation; Acceleration; Adaptive Immune System; Adrenergic Receptor; Anatomy; Animals; Area; Bacterial Infections; Blood capillaries; Brain; CD8-Positive T-Lymphocytes; Cell physiology; Cell secretion; Cells; Characteristics; Circadian Rhythms; Circulation; Communication; Cre lox recombination system; Cytomegalovirus; Data; Dependovirus; Development; Diabetes Mellitus; Diabetic Retinopathy; Diabetic mouse; Diphtheria Toxin; Disease; Electrophysiology (science); Enteral; Environmental Risk Factor; Excision; Flow Cytometry; Functional disorder; Genetic; Health; Homeostasis; Host Defense; Hyperactivity; Hypothalamic dysfunction; Hypothalamic structure; IL17 gene; Immune; Impairment; Inflammation; Inflammatory; Injections; Injury; Innate Immune System; Insulin-Dependent Diabetes Mellitus; Intestines; Mediating; Modeling; Mus; Mycoses; Nerve; Neurons; Neurotransmitters; Octreotide; Output; Pathogenesis; Pathogenicity; Pathologic; Phenotype; Plasma; Population; Property; Publishing; Regulatory T-Lymphocyte; Reporter; Retina; Role; Serum; Signal Transduction; Site; Somatostatin; Streptozocin; Synapses; System; T-Lymphocyte; T-Lymphocyte Subsets; Testing; Tissues; Viral; cell motility; comparison control; cytokine; cytotoxic; designer receptors exclusively activated by designer drugs; diabetic; dysbiosis; gain of function; gut microbiome; immune function; immunoregulation; intestinal barrier; loss of function; microbiota; microorganism antigen; migration; monocyte; non-diabetic; novel strategies; optogenetics; paraventricular nucleus; peripheral blood; pharmacologic; preservation; prevent; programs; recruit; response; single-cell RNA sequencing; small hairpin RNA; somatostatin analog; tissue injury; tool; trafficking; transcriptome sequencing

Summary Abstract This application puts for the notion of a brain-gut- retinal axis that becomes dysfunctional in diabetic retinopathy (DR). The innate immune system has been strongly implicated in the pathogenesis of DR, but less is known about the role of the adaptive immune system. At the interface of these two systems is a critical population of cells, Th17 cells, that typically reside in the gut during health. Th17 cells have homeostatic properties, mediating host defense against bacterial and fungal infections; however, it remains unclear how intestinal Th17 cells integrate diverse signals into a set of cellular programs that allow them to maintain tissue homeostasis yet also become pathogenic, serving as primary drivers of tissue inflammation. We have identified a critical role of somatostatinergic neurons in the paraventricular nucleus of the hypothalamus (PVN) in regulation of immune function through “loss of function” studies and “gain of function studies. SST expression is dramatically reduced in the PVN of diabetic animals. Hypothalamic dysfunction, as seen in diabetes, has the capacity to induce injury directly through hyperactivation of sympathetic nerves. Based on preliminary and published data, we put for the hypothesis that: Diabetes-induced loss of inhibitory SST neurons in the PVN drives increased autonomic input to the intestine shifting Th17 cells from a homeostatic to a pathologic state. Pathologic Th17 cells leave the intestine and traffic to areas of tissue injury such as the retina in DR. In the retina the pathologic Th17 cells secrete proinflammatory cytokines that recruitment innate immune cells into the retina exacerbating DR. To examine this hypothesis, we propose the following aims: Aim 1: To test if impaired function of hypothalamic SST neurons in diabetes contributes to hyperactivity of autonomic efferents to the gut and increases activation of enteric neurons. Aim 2: To selectively ablate SST PVN neurons (in the absence of diabetes) and evaluate if this results in increased autonomic input to the gut and a shift from “homeostatic” Th17 cells to a “pathogenic” Th17 cells that migrate to the retina and recruit circulating immune cells. Aim 3: To determine if maintaining hypothalamic SST levels at nondiabetic levels in diabetic mice will preserve the function of homeostatic Th17 cells in the gut and prevent their recruitment to the retina delaying the development of DR. Impact: SST analogues may provide an important complementary strategy for DR management by preventing increased sympathetic drive to the gut and Th17 cell dysfunction.

摘要摘要 这个应用程序支持大脑-肠道-视网膜轴的概念，糖尿病患者的大脑-肠道-视网膜轴功能障碍 视网膜病变(DR)。先天免疫系统与DR的发病机制密切相关， 但人们对适应性免疫系统的作用知之甚少。在这两个系统的接口处 一种关键的细胞群，Th17细胞，通常在健康期间驻留在肠道中。Th17细胞有 动态平衡特性，调节宿主对细菌和真菌感染的防御；然而，它 目前尚不清楚肠道Th17细胞如何将不同的信号整合到一系列细胞程序中 允许它们维持组织的动态平衡，但也会成为致病的，作为主要的驱动因素 组织发炎。我们已经确定了生长抑素能神经元在脑室旁的关键作用。 下丘脑核(PVN)通过“功能丧失”研究调节免疫功能 和“功能研究的收获”。糖尿病动物室旁核中SST的表达显著降低。 下丘脑功能障碍，如糖尿病患者，有能力直接通过 交感神经过度活跃。根据初步和公布的数据，我们提出了 假设：糖尿病引起的下丘脑室旁核SST抑制神经元的丢失增加了自主神经 进入肠道，将Th17细胞从内环境平衡状态转变为病理状态。病理性Th17细胞 离开肠道和交通到组织损伤的区域，例如视网膜。 病理性Th17细胞分泌促炎细胞因子，募集先天免疫细胞进入 视网膜恶化博士为了检验这一假说，我们提出了以下目标：目标1：检验 糖尿病患者下丘脑SST神经元功能受损导致自主神经功能亢进 传出到肠道，并增加肠道神经元的激活。目的2：选择性消融SST室旁核 神经元(在没有糖尿病的情况下)，并评估这是否会导致肠道自主神经输入增加 从“内稳态”的Th17细胞转变为“致病的”Th17细胞，这种细胞迁移到视网膜和 招募循环免疫细胞。目标3：确定是否将下丘脑SST水平维持在 糖尿病小鼠体内的非糖尿病水平将保护肠道内平衡的Th17细胞的功能 阻止它们重新聚集到视网膜，延缓Dr.Impact的发展：SST类似物可能 通过防止交感神经增加，为DR管理提供重要的补充策略 驱使肠道和Th17细胞功能障碍。