MetXtra - An Integrated High-Throughput Metagenomics Platform for Enzyme Discovery

MetXtra - 用于酶发现的集成高通量宏基因组学平台

• 批准号: 102513
• 金额: $ 32.7万
• 依托单位: BIOCATALYSTS LIMITED
• 依托单位国家: 英国
• 项目类别: Collaborative R&D
• 财政年份: 2016
• 资助国家: 英国
• 起止时间: 2016 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=102513
• 关键词: MetXtra; Integrated; Throughput; Metagenomics; Platform

Only a tiny fraction (~1%) of all microorganisms can currently be grown in the laboratory and hence have theirDNA sequenced. Thus, there is a huge wealth of genetic sequence data still to be discovered fromenvironmental samples of the remaining 99% of uncultured microorganisms. The field of metagenomicsdirectly samples microorganisms living in the environment, thereby avoiding the need for laboratory culturingbefore DNA sequencing. Metagenomics projects can yield very large amounts of DNA sequence data and themajor bottleneck to exploiting this data for enzyme discovery is the actual data analysis. The objective of thisproject between Biocatalysts Ltd and EMBL-EBI is to develop a novel and unique data analysis softwareplatform, MetXtra, for the rapid and efficient identification of completely novel enzymes for IndustrialBiotechnology (IB) applications from large metagenomic DNA sequence libraries.

目前，所有微生物中只有一小部分(~1%)可以在实验室中培养，因此可以对它们的DNA进行测序。因此，从剩余99%的未培养微生物的环境样本中仍有大量的遗传序列数据有待发现。元基因组学领域直接对生活在环境中的微生物进行采样，从而避免在DNA测序之前进行实验室培养。元基因组学项目可以产生非常大量的DNA序列数据，而利用这些数据进行酶发现的主要瓶颈是实际数据分析。Biocatalysts Ltd和EMBL-EBI之间的这个项目的目标是开发一个新颖而独特的数据分析软件平台MetXtra，用于从大型元基因组DNA序列库中快速高效地识别用于工业生物技术(IB)的全新酶。