SALIVARY SECRETION IN A BLOODSUCKING ARTHROPOD

吸血节肢动物的唾液分泌

• 批准号: 2063261
• 负责人: JOHN ROBERT SAUER
• 金额: $ 12.97万
• 依托单位: OKLAHOMA STATE UNIVERSITY STILLWATER
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-04-01 至 1996-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2063261
• 关键词: G protein; biological signal transduction; cyclic AMP; dopamine; dopamine receptor; eating; enzyme activity; high performance liquid chromatography; laboratory rabbit; molecular chaperones; nucleic acid sequence; phosphatidylcholines; phosphatidylinositols; phosphorylation; polymerase chain reaction; protein kinase C; protein tyrosine kinase; receptor binding; salivary glands; salivation; sheep; thin layer chromatography; ticks

The salivary glands and their secretions are vital to the capacity of ixodid ticks to he parasitic and are the major route by which pathogenic organisms and toxins access the vertebrate host. We propose to continue research on the characterization of receptors and signal pathways controlling salivary gland function in an ixodid tick, Amblyomma americanum (L.). The evidence implicating dopamine as a neurotransmitter at the neuroeffector junction and its interaction with a D-1 receptor is compelling; however, the effects of dopamine receptor antagonists on dopamine stimulated fluid secretion suggest that dopamine control is more complex and may utilize multiple receptors. Receptor binding studies, cyclic AMP, and secretion assays will he used to clarify the role of dopamine and dopamine receptors in the salivary glands. Other data indicates the existence of a ligand (neuropeptide from the tick synganglion), which through a receptor mediated interaction, activates phospholipase C (PLC) catalyzed hydrolysis of plasma membrane phosphoinositides. We propose to study the forms and characteristics of PLC. We will also address the question of whether PLC is activated through a receptor tyrosine kinase or GTP-dependent protein. Signalling through phosphatidylcholine breakdown and its relationship, if any, to phosphoinositide turnover will also be investigated. Since salivary secretion is regulated by protein kinase-catalyzed phosphorylation of proteins, we will continue ongoing molecular characterization of protein kinases and their role in controlling salivary gland function. Information about receptors and regulation of cell functions in the salivary glands will provide key insights into factors which enable ticks to be parasitic and their relationship to pathogen transmission to hosts.

唾液腺和它们的分泌物对于 硬蜱是寄生性的，是致病性蜱的主要途径。 微生物和毒素进入脊椎动物宿主。 我们建议继续研究受体的特性， 在硬蜱中控制唾液腺功能的信号通路， 美洲钝眼藓Amblyomma americanum（L.）证据表明多巴胺是一种 神经效应器接头处的神经递质及其与 D-1受体是引人注目的;然而，多巴胺受体的作用， 拮抗剂对多巴胺刺激的液体分泌表明，多巴胺 控制更复杂，并且可以利用多种受体。受体 结合研究，环腺苷酸，和分泌测定将被用来澄清 多巴胺和多巴胺受体在唾液腺中的作用。 其他数据表明存在配体（来自蜱的神经肽 突触神经节），通过受体介导的相互作用，激活 磷脂酶C（PLC）催化质膜水解 磷酸肌醇。我们建议研究的形式和特点 PLC.我们亦会讨论临立会是否透过 受体酪氨酸激酶或GTP依赖性蛋白。信号通过 磷脂酰胆碱分解及其与 还将研究磷酸肌醇周转。 由于唾液分泌是由蛋白激酶催化的 蛋白质的磷酸化，我们将继续进行分子 蛋白激酶的表征及其在控制唾液分泌中的作用 腺体功能 关于受体和细胞功能调节的信息， 唾液腺将提供关键的洞察因素，使蜱 是寄生性的，以及它们与病原体向宿主传播的关系。