CD8+ CTL RECOGNITION OF MHC CLASS I
MHC I 类的 CD8 CTL 识别
基本信息
- 批准号:2063944
- 负责人:
- 金额:$ 18.73万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1989
- 资助国家:美国
- 起止时间:1989-09-30 至 1999-11-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
DESCRIPTION (Adapted from the Applicant's abstract): The T-cell receptor
(TCR) and its coreceptor CD8 are used by cytotoxic T lymphocytes (CTL)
to identify virus-infected or malignant target cells. Such target cells
express class I MHC molecules with bound peptide ligands that are
specifically recognized by the TCR/CD8 complex. The work proposed here
will investigate several aspects of the molecular interaction of TCR/CD8
with class I/peptide. It has been recently determined that a single
peptide is immunodominant in Ld- allorecognition. This observation will
be extended to determine if this immunodominance is reflected in the
quantitative expression of this peptide, the affinity of the peptide for
Ld, or the affinity of the TCR for the Ld/peptide complex. As an
extension of earlier studies, this laboratory has developed a method to
generate peptide-specific alloreactive CTL to H-2Ld. Using this
methodology, peptide-specific alloreactive CTL will be generated to
known endogenous Ld ligands to determine whether they function in
allogeneic responses and whether their expression is ubiquitous on
different cell types. We will also test whether allogeneic CTL, specific
for endogenous Ld ligands, are more peptide cross-reactive than
syngeneic CTL specific for viral peptide ligands. To better understand
how specific TCR structural motifs interact with class I or its bound
ligand, an extensive panel of site-specific mutants of class I will be
subjected to structure-function analyses. These studies will also
include several CTL clones restricted by the same class I but specific
for different peptides as well as CTL clones restricted by similar class
I molecules and specific for the same peptide. As an extension of
earlier studies mapping the CD8 recognition site, a class I alpha-3
mutant not recognized by CD8, will be characterized in vivo and in vitro
to better define the role of CD8 in the induction of CTL responses and
in class I recognition by CTL. In summary, the proposed experiments
will exploit unique resources and approaches to define various
parameters governing functional interactions of the TCR/CD8 molecules
on CTL with class I/peptide molecules on target cells.
描述(改编自申请人摘要):T细胞受体
(TCR)细胞毒性T淋巴细胞(CTL)利用其辅助受体CD 8
以识别病毒感染或恶性靶细胞。这样的靶细胞
表达具有结合的肽配体的I类MHC分子,
由TCR/CD 8复合物特异性识别。这里提出的工作
将研究TCR/CD 8分子相互作用的几个方面
I类/肽。最近已经确定,
肽在Ld-同种异体识别中是免疫显性的。这一观察将
扩展以确定这种免疫优势是否反映在
定量表达该肽,测定肽对
Ld,或TCR对Ld/肽复合物的亲和力。作为
在早期研究的基础上,该实验室开发了一种方法,
产生针对H-2Ld的肽特异性同种异体反应性CTL。使用此
方法,将产生肽特异性同种异体反应性CTL,
已知的内源性Ld配体,以确定它们是否在
同种异体反应,以及它们的表达是否在
不同的细胞类型我们还将测试同种异体CTL,特异性
对于内源性Ld配体,比内源性Ld配体更具有肽交叉反应性。
对病毒肽配体具有特异性同基因CTL。更好地了解
特异性TCR结构基序如何与I类或其结合物相互作用
配体,一个广泛的小组的位点特异性突变的I类将是
进行结构功能分析。这些研究还将
包括几个受相同I类限制但特异性
对于不同的肽以及受类似类别限制的CTL克隆,
I分子和特异性肽相同。的延伸
早期的研究绘制了CD 8识别位点,一个I类α-3
不被CD 8识别的突变体,将在体内和体外表征
为了更好地确定CD 8在诱导CTL应答中的作用,
在CTL的I类识别中。总之,所提出的实验
将利用独特的资源和方法来定义各种
控制TCR/CD 8分子的功能性相互作用的参数
对CTL与靶细胞上的I类/肽分子的作用。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
JANET M CONNOLLY其他文献
JANET M CONNOLLY的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('JANET M CONNOLLY', 18)}}的其他基金
CD8-LYT-2 RECOGNITION OF THE CLASS I ALPHA 3 DOMAIN
CD8-LYT-2 对 I 类 ALPHA 3 结构域的识别
- 批准号:
3141829 - 财政年份:1989
- 资助金额:
$ 18.73万 - 项目类别:
CD8-LYT-2 RECOGNITION OF THE CLASS I ALPHA 3 DOMAIN
CD8-LYT-2 对 I 类 ALPHA 3 结构域的识别
- 批准号:
3141824 - 财政年份:1989
- 资助金额:
$ 18.73万 - 项目类别:
相似海外基金
Elucidating a molecular understanding of ILC2 MHC class I antigen cross priming
阐明 ILC2 MHC I 类抗原交叉引发的分子理解
- 批准号:
486527 - 财政年份:2022
- 资助金额:
$ 18.73万 - 项目类别:
Studentship Programs
Restoring MHC class I antigen presentation to enhance anti-tumour immunity
恢复MHC I类抗原呈递,增强抗肿瘤免疫力
- 批准号:
nhmrc : GNT1164054 - 财政年份:2020
- 资助金额:
$ 18.73万 - 项目类别:
Project Grants
Subversion of MHC class I antigen presentation by viral immunomodulatory proteins
病毒免疫调节蛋白颠覆 MHC I 类抗原呈递
- 批准号:
8996707 - 财政年份:2014
- 资助金额:
$ 18.73万 - 项目类别:
Ubiquitin conjugation and direct MHC class I antigen presentation
泛素结合和直接 MHC I 类抗原呈递
- 批准号:
8880646 - 财政年份:2014
- 资助金额:
$ 18.73万 - 项目类别:
Subversion of MHC class I antigen presentation by viral immunomodulatory proteins
病毒免疫调节蛋白颠覆 MHC I 类抗原呈递
- 批准号:
9206412 - 财政年份:2014
- 资助金额:
$ 18.73万 - 项目类别:
Subversion of MHC class I antigen presentation by viral immunomodulatory proteins
病毒免疫调节蛋白颠覆 MHC I 类抗原呈递
- 批准号:
8723605 - 财政年份:2014
- 资助金额:
$ 18.73万 - 项目类别:
MHC Class I Antigen Presentation in Viral Infections
病毒感染中 MHC I 类抗原呈递
- 批准号:
8072956 - 财政年份:2010
- 资助金额:
$ 18.73万 - 项目类别:
The function of the Ubiquitin-Proteasome-System (UPS) in MHC class I antigen processing in target cells and maturing human dendritic cells (hDCs).
泛素蛋白酶体系统 (UPS) 在靶细胞和成熟人树突细胞 (hDC) 中 MHC I 类抗原加工中的功能。
- 批准号:
105348415 - 财政年份:2009
- 资助金额:
$ 18.73万 - 项目类别:
Research Grants
In vivo analysis of the imnportance of proteasome immunosubunits and of PA28 for MHC class I antigen processing, CTL response induction and tumor-virus elimination (A 7)
体内分析蛋白酶体免疫亚基和 PA28 对于 MHC I 类抗原加工、CTL 反应诱导和肿瘤病毒消除的重要性 (A 7)
- 批准号:
5354871 - 财政年份:2002
- 资助金额:
$ 18.73万 - 项目类别:
Collaborative Research Centres
MHC Class I Antigen Presentation in Viral Infections
病毒感染中 MHC I 类抗原呈递
- 批准号:
7991835 - 财政年份:2001
- 资助金额:
$ 18.73万 - 项目类别: