课题基金 / 基金详情

NADPH-BINDING PROTEIN OF THE RESPIRATORY BURST OXIDASE

NADPH-BINDING PROTEIN OF THE RESPIRATORY BURST OXIDASE
呼吸爆发氧化酶的 NADPH 结合蛋白
批准号：
2065866
负责人：
Bernard M Babior
金额：
$24.78万
依托单位：
SCRIPPS RESEARCH INSTITUTE, THE
依托单位国家：
美国
项目类别：
财政年份：
1991
资助国家：
美国
项目状态：
已结题
起止时间：
1991-01-01 至 1995-12-31
来源：
https://reporter.nih.gov/project-details/2065866
关键词：
NAD(H) phosphate SDS polyacrylamide gel electrophoresis affinity chromatography affinity labeling atomic absorption spectrometry binding proteins cell free system cell membrane chronic granulomatous disease complementary DNA electron spin resonance spectroscopy enzyme induction /repression enzyme linked immunosorbent assay enzyme reconstitution enzyme structure high performance liquid chromatography human subject hybridomas immunoprecipitation ion exchange chromatography laboratory mouse laboratory rabbit leukocyte oxidative burst molecular cloning molecular genetics monoclonal antibody neutrophil nucleic acid sequence polymerase chain reaction protein purification respiratory enzyme southern blotting western blottings

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中文摘要
翻译
呼吸爆发氧化酶是负责
英文摘要
The respiratory burst oxidase is the enzyme responsible for the production of O2- by stimulated phagocytes. A highly complex enzyme composed of many component parts, it catalyzes the oneelectron reduction of oxygen to O 2- at the expense of NADPH. One of its components is an NADPH-binding polypeptide that is found in the cytosol of resting neutrophils but is transferred to the plasma membrane when the cells are activated, thereby assembling the electron transport chain of the enzyme. The goals of the project described in this application are the purification and characterization of this NADPH-binding component and the study of its interaction with other components of the oxidase. Through this work it may ultimately be possible to develop a sufficiently detailed molecular understanding of the operation of the respiratory burst oxidase to allow the development of a new class of anti-inflammatory agents that work by specifically controlling the production of reactive oxidants by phagocytes.
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Function of normal and malignant phagocytes
  • 批准号：
    7042993
  • 项目类别：
  • 资助金额：
    $4.33万
  • 财政年份：
    2004
  • 负责人：
    Bernard M Babior
  • 依托单位：
    SCRIPPS RESEARCH INSTITUTE, THE
P56C2, A NOVEL C2 DOMAIN PROTEIN OF LEUKOCYTES
  • 批准号：
    2761192
  • 项目类别：
  • 资助金额：
    $33.39万
  • 财政年份：
    1998
  • 负责人：
    Bernard M Babior
  • 依托单位：
    SCRIPPS RESEARCH INSTITUTE, THE
P56C2, A NOVEL C2 DOMAIN PROTEIN OF LEUKOCYTES
  • 批准号：
    6124282
  • 项目类别：
  • 资助金额：
    $34.39万
  • 财政年份：
    1998
  • 负责人：
    Bernard M Babior
  • 依托单位：
    SCRIPPS RESEARCH INSTITUTE, THE
P56C2, A NOVEL C2 DOMAIN PROTEIN OF LEUKOCYTES
  • 批准号：
    6328813
  • 项目类别：
  • 资助金额：
    $35.42万
  • 财政年份：
    1998
  • 负责人：
    Bernard M Babior
  • 依托单位：
    SCRIPPS RESEARCH INSTITUTE, THE