NADPH-BINDING PROTEIN OF THE RESPIRATORY BURST OXIDASE

呼吸爆发氧化酶是负责 受刺激的吞噬细胞产生O2-。 一种高度复杂的酶 它由许多组分组成，催化单电子还原反应， 以NADPH为代价将氧气转化为O2。 它的一个组成部分是 在静息细胞胞浆中发现的NADPH结合多肽 中性粒细胞，但转移到质膜时，细胞是 激活，从而组装酶的电子传递链。 本申请中描述的项目目标是 这种NADPH结合组分的纯化和表征， 研究其与氧化酶的其他组分的相互作用。 通过 这项工作最终可能会开发出一个充分的 详细了解呼吸系统的分子运作， 爆发氧化酶，使一类新的发展， 抗炎剂通过特异性地控制 吞噬细胞产生反应性氧化剂。

The respiratory burst oxidase is the enzyme responsible for the production of O2- by stimulated phagocytes. A highly complex enzyme composed of many component parts, it catalyzes the oneelectron reduction of oxygen to O 2- at the expense of NADPH. One of its components is an NADPH-binding polypeptide that is found in the cytosol of resting neutrophils but is transferred to the plasma membrane when the cells are activated, thereby assembling the electron transport chain of the enzyme. The goals of the project described in this application are the purification and characterization of this NADPH-binding component and the study of its interaction with other components of the oxidase. Through this work it may ultimately be possible to develop a sufficiently detailed molecular understanding of the operation of the respiratory burst oxidase to allow the development of a new class of anti-inflammatory agents that work by specifically controlling the production of reactive oxidants by phagocytes.