NADPH-BINDING PROTEIN OF THE RESPIRATORY BURST OXIDASE

呼吸爆发氧化酶是一种负责 刺激吞噬细胞产生O2-。一种高度复杂的酶 它由许多组分组成，催化单电子还原。 以NADPH为代价将氧气转化为O2-。它的组件之一是一个 在休眠的胞浆中发现的NADPH结合多肽 中性粒细胞，但当细胞 激活，从而组装酶的电子传输链。 此应用程序中描述的项目的目标是 NADPH结合组分的纯化和鉴定 研究其与酶的其他成分的相互作用。穿过 这项工作最终可能开发出一种充分的 对呼吸系统的操作有详细的分子理解 猝发氧化物酶允许开发一类新的 抗炎剂通过特别控制 吞噬细胞产生活性氧化剂。

The respiratory burst oxidase is the enzyme responsible for the production of O2- by stimulated phagocytes. A highly complex enzyme composed of many component parts, it catalyzes the oneelectron reduction of oxygen to O 2- at the expense of NADPH. One of its components is an NADPH-binding polypeptide that is found in the cytosol of resting neutrophils but is transferred to the plasma membrane when the cells are activated, thereby assembling the electron transport chain of the enzyme. The goals of the project described in this application are the purification and characterization of this NADPH-binding component and the study of its interaction with other components of the oxidase. Through this work it may ultimately be possible to develop a sufficiently detailed molecular understanding of the operation of the respiratory burst oxidase to allow the development of a new class of anti-inflammatory agents that work by specifically controlling the production of reactive oxidants by phagocytes.