GENETICS AND BIOGEOGRAPHY OF SANDFLY DISEASE VECTORS

这个项目的长期目标是研究遗传学和 白纹伊蚊的生物地理学研究(双翅目：心蝇科)小才是 了解这一重要昆虫群体的遗传学，其成员 作为利什曼病、巴尔通氏杆菌病和一些虫媒病毒的媒介 疾病。初步研究将集中在Lutzmyia long gipalpis和L. 香诺尼，两个被牵连为传播媒介的物种 利什曼病，在美洲有非常广泛的地理范围。 拟议的项目包括实地研究和实验室研究。 Long gipalpis和L.shannoni的代表性野外收藏将是 在整个地理范围内形成的；生化的和形态的 这两个物种的检索表将被开发出来，以及不同的地理位置 它们的分布参数。每个集合的基因图谱 将使用酶电泳法构建。独特的地理位置和 还将比较长瓣乳杆菌的遗传种群及其 对病原体查加西利什曼原虫感染的易感性 新大陆内脏利什曼病。基因图谱将被构建为 两个医学上重要的沙蝇属(Lutzmyia和Phlebotomus)， 基于生化标记，染色体数目，重组 频率和连锁图。后一项工作将利用实验室 在耶鲁虫媒病毒研究中心维护的沙蝇群落。 该项目是耶鲁大学的科学家们共同努力的成果 哥伦比亚波哥大的大学和国家萨卢德研究所，但 美国和拉丁美洲的其他沙蝇专家将 协助田野收集工作。

The long-term objective of this project is to study the genetics and biogeography of phlebotomine sand flies (Diptera: psychodidae). Little is known about the genetics of this important insect group, members of which serve as vectors of leishmaniasis, bartonellosis and a number of arboviral diseases. Initial studies will focus on Lutzomyia longipalpis and L. shannoni, two species which have been implicated as vectors of leishmaniasis and which have a very wide geographic range in the Americas. The proposed project involves both field and laboratory studies. Representative field collections of L. longipalpis and L. shannoni will be made throughout their geographic ranges; biochemical and morphological keys to both species will be developed as well as various geographic parameters of their distribution. Genetic profiles for each collection will be constructed using enzyme electrophoresis. Distinct geographic and genetic populations of L. longipalpis will also be compared for their susceptibility to infection with Leishmania chagasi, the etiologic agent of New World visceral leishmaniasis. Genetic maps will be constructed for the two medically important sand fly genera (Lutzomyia and Phlebotomus), based on biochemical markers, chromosome numbers, recombination frequencies and linkage maps. The latter work will utilize laboratory colonies of sand flies maintained at the Yale Arbovirus Research Unit. The project is a collaborative effort between scientists at Yale University and the Instituto Nacional de Salud in Bogota, Colombia, but a number of other sand fly specialists in the U.S. and Latin America will assist with the field collections.