TYPES OF DIETARY FAT AND COLON CANCER

长期目标是了解类型的调节效果 脂肪酸组成不同的饮食脂肪对结肠癌的影响。 具体地说，建议研究类风湿性关节炎的发病机制(S)。 结肠脂肪含量，即玉米油(CO)和鱼油(FO) 致癌。偶氮甲烷(AOM)，一种脂肪族偶氮化合物 用来诱发结肠肿瘤。日粮种类和用量的影响 脂肪对与结肠相关的生化参数的影响 肿瘤促进作用将被确定，并将包括盲肠细菌 7α-脱羟基酶和磷脂酶C的活性，粪便中的二甘油 胆汁酸，结肠粘膜和肿瘤，1，2-二酰甘油， 磷脂酶C，DAG激酶，蛋白激酶C，前列腺素合成， 花生四烯酸通过环氧合酶途径和ras p21的代谢。 5周龄雄性F344大鼠饲喂低脂AIN-76A饲料。在… 7周龄，每组大鼠每周接受两次皮下注射。剂量 AOM或生理盐水(载体)，并转移到高脂肪饮食 含CO或FO饮食。然后，在AOM之后的第1天和第1周和第12周 治疗和终止时(Wk 42)，上述生化指标 将在结肠粘膜和肿瘤中进行测量(在终止时)以阐明 类型和数量在结肠癌发生中的作用机制。另外， 在4周和12周的试验性饮食和终止妊娠期间， 来自每个饮食组的动物分组将被AOM处理 处死盲肠细菌7α-脱羟基酶和磷脂酶C 粪便胆汁酸和二甘油三酯也会被检测出来。生化 并对不同饮食组的生理参数进行分析 经统计学处理，确定差异有统计学意义。选定的 把生化参数放在一起会显示出可能的 不同膳食类型对体内肿瘤促进或抑制机制的研究 胖的。

The long-term objective is to understand the modulating effect of types of dietary fat differing in fatty acid composition on colon cancer. Specifically, it is proposed to study the mechanism(s) of types and amount of fat namely corn oil (CO) and menhaden fish oil (FO) on colon carcinogenesis. Azoxymethane (AOM), an aliphatic azoxy compound will be used to induce colon tumors. The effect of types and amount of dietary fat on the relevant biochemical parameters that are associated with colon tumor promotion will be determined and will include cecal bacterial 7alpha-dehydroxylase and phospholipase C activities, fecal diglycerides and bile acids, and colonic mucosal and tumor, 1,2-diacylglycerol, phospholipase C, DAG kinase, protein kinase C, prostaglandin synthesis, arachidonic acid metabolism via cyclooxygenase pathway, and ras p21. At 5 weeks of age, male F344 rats will be fed low-fat AIN-76A diet. At 7 weeks of age, groups of rats will be treated with two weekly s.c. doses of AOM or normal saline (vehicle) and transferred to high fat diets containing CO or FO diet. Then, day at 1 and week 1 and 12 following AOM treatment and at termination (wk 42), the above biochemical parameters will be measured in colon mucosa and tumors (at termination) to elucidate the mechanistic role of types and amount in colon carcinogenesis. Also, during the weeks of 4 and 12 on experimental diets and at termination, groups of animals from each dietary group treated with AOM will be sacrificed and cecal bacterial 7alpha-dehydroxylase and phospholipase C and fecal bile acids and diglycerides will be determined. Biochemical and physiological parameters of various dietary groups will be analyzed statistically to determine the significance of difference. The selected biochemical parameters taken together would suggest the possible mechanism of in vivo tumor promotion or inhibition by types of dietary fat.