TYPES OF DIETARY FAT AND COLON CANCER

长期目标是了解类型的调节作用 不同脂肪酸组成的膳食脂肪对结肠癌的影响。 具体而言，建议研究类型和 结肠上的脂肪量，即玉米油（CO）和鲱鱼鱼油（FO 致癌作用 氧化偶氮甲烷（AOM），一种脂肪族氧化偶氮化合物， 用来诱发结肠肿瘤 不同类型和数量的日粮 脂肪对结肠相关生化参数的影响 将确定肿瘤促进，并将包括盲肠细菌 7 α-脱羟酶和磷脂酶C活性，粪便甘油二酯 和胆汁酸，和结肠粘膜和肿瘤，1，2-二酰基甘油， 磷脂酶C，DAG激酶，蛋白激酶C，前列腺素合成， 花生四烯酸通过环氧合酶途径的代谢和ras p21。 在5周龄时，雄性F344大鼠将喂食低脂AIN-76 A饮食。 在 7周龄时，各组大鼠将用每周两次s.c.剂量 AOM或生理盐水（溶媒），并转移至高脂肪饲料 含CO或FO饮食。 然后，在AOM后第1天、第1周和第12周 治疗和终止时（wk 42），上述生化参数 将在结肠粘膜和肿瘤（处死时）中进行测量以阐明 类型和数量在结肠癌发生中的机制作用。 还有， 在实验饮食的第4周和第12周期间以及在终止时， 将来自用AOM处理的每个饮食组的动物组 处死和盲肠细菌7 α-脱羟酶和磷脂酶C 并测定粪便胆汁酸和甘油二酯。 生化 分析不同膳食组的生理参数 统计学上确定差异的显著性。 所选 生化参数综合起来表明 通过饮食类型的体内肿瘤促进或抑制的机制 胖了

The long-term objective is to understand the modulating effect of types of dietary fat differing in fatty acid composition on colon cancer. Specifically, it is proposed to study the mechanism(s) of types and amount of fat namely corn oil (CO) and menhaden fish oil (FO) on colon carcinogenesis. Azoxymethane (AOM), an aliphatic azoxy compound will be used to induce colon tumors. The effect of types and amount of dietary fat on the relevant biochemical parameters that are associated with colon tumor promotion will be determined and will include cecal bacterial 7alpha-dehydroxylase and phospholipase C activities, fecal diglycerides and bile acids, and colonic mucosal and tumor, 1,2-diacylglycerol, phospholipase C, DAG kinase, protein kinase C, prostaglandin synthesis, arachidonic acid metabolism via cyclooxygenase pathway, and ras p21. At 5 weeks of age, male F344 rats will be fed low-fat AIN-76A diet. At 7 weeks of age, groups of rats will be treated with two weekly s.c. doses of AOM or normal saline (vehicle) and transferred to high fat diets containing CO or FO diet. Then, day at 1 and week 1 and 12 following AOM treatment and at termination (wk 42), the above biochemical parameters will be measured in colon mucosa and tumors (at termination) to elucidate the mechanistic role of types and amount in colon carcinogenesis. Also, during the weeks of 4 and 12 on experimental diets and at termination, groups of animals from each dietary group treated with AOM will be sacrificed and cecal bacterial 7alpha-dehydroxylase and phospholipase C and fecal bile acids and diglycerides will be determined. Biochemical and physiological parameters of various dietary groups will be analyzed statistically to determine the significance of difference. The selected biochemical parameters taken together would suggest the possible mechanism of in vivo tumor promotion or inhibition by types of dietary fat.