Production of difficult to express essential bacterial proteins

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Absynth Biologics (http://www.absynthbiologics.co.uk) has a novel platform of protein antigens for prevention of bacterial infections. Absynth's approach is a means of fighting the increase in antibiotic resistance by preventing infections and therefore directly reducing antibiotic use. Having already demonstrated its approach can work in principle, a key step for Absynth is the production of its proteins (or variants of them) efficiently and cost-effectively, so that they can be made to a consistent quality at an economic cost. This is where the expertise of the University of Manchester is important and forms the basis for the shared programme in this FLIP project. The University of Manchester, through the considerable expertise built up by Dr Eddie McKenzie and the Protein Expression Faciility (competencies that have been tested tested by successful optimsiation of expression of proteins in multiple academic and industrial collaborative project,) has proven knowledge and technical skills to develop efficient processes to produce the Absynth proteins, which will be tested to further optimize their use in preventing infection. The research project will see the proteins from Absynth being put through the unique profiling technology developed at the University of Manchester. The FLIP interchanger scientist (Ms Hirra Hussain) will undertake this work at both at the University of Manchester (6-7 months) and at Absynth's laboratories (5-6 months). The programme described presents a mutally beneficial exchange of knowledge and technologies (embedded into the work programme by a scientist who will work at both sites). Success in the project outlined will provide an exemplar approach for Absynth to expand into other projects and for the University of Manchester to apply its improved understanding of fundamental production mechanisms to other related molecules.