FUNCTIONAL ANALYSIS OF ADIPOCYTE LIPID BINDING PROTEIN

脂肪细胞脂质结合蛋白的功能分析

• 批准号: 2136394
• 负责人: ELIZABETH R SMITH
• 金额: $ 1.14万
• 依托单位: RUTGERS, THE STATE UNIV OF N.J.
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-03-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2136394
• 关键词: FUNCTIONAL; ANALYSIS; ADIPOCYTE; LIPID; BINDING

The goal of the proposed research is to determine the function of the adipocyte fatty acid binding protein (AFABP) in the transport and metabolism of fatty acids in adipose tissue. Fatty acid trafficking is central to the ability of adipose tissue to store and mobilize energy, and imbalances in the control of fatty acid metabolism accompany obesity, insulin resistance, and diabetes. AFABP is believed, but have never been directly proven, to be centrally important in the transport of fatty acids. This study utilizes transgenic mice in which the AFABP gene has been disrupted by homologous recombination and which lack the AFABP protein. Given the abundance and tissue-specificity of AFABP, the absence of the protein should alter adipocyte lipid homeostasis. Using the AFABP-knockout mice, the proposed research will investigate the role of AFABP in fatty acid transport into and within adipocytes, as well as in the regulation of white and brown adipocyte lipid metabolism.

拟议研究的目标是确定 脂肪细胞脂肪酸结合蛋白（AFABP）在运输和 脂肪组织中脂肪酸的代谢。 脂肪酸运输是 对脂肪组织储存和动员能量的能力至关重要， 肥胖伴随着脂肪酸代谢的控制失衡， 胰岛素抵抗和糖尿病。 AFABP 被相信，但从未被相信 直接证明，对于脂肪的运输至关重要 酸。 本研究利用转基因小鼠，其中 AFABP 基因具有 被同源重组破坏且缺乏 AFABP 蛋白质。 鉴于 AFABP 的丰度和组织特异性， 蛋白质的缺失会改变脂肪细胞脂质稳态。 使用 AFABP 基因敲除小鼠，拟议的研究将调查其作用 AFABP 在脂肪酸转运到脂肪细胞内和脂肪细胞内的过程中的作用，以及 调节白色和棕色脂肪细胞的脂质代谢。