FUNCTIONAL ANALYSIS OF ADIPOCYTE LIPID BINDING PROTEIN

脂肪细胞脂质结合蛋白的功能分析

• 批准号: 2634173
• 负责人: ELIZABETH R SMITH
• 金额: $ 3.05万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-12-08 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2634173
• 关键词: adipocytes; fatty acid binding protein; fatty acid metabolism; fatty acid transport; obesity

The goal of the proposed research is to determine the function of the adipocyte fatty acid binding protein (AFABP) in the transport and metabolism of fatty acids in adipose tissue. Fatty acid trafficking is central to the ability of adipose tissue to store and mobilize energy, and imbalances in the control of fatty acid metabolism accompany obesity, insulin resistance, and diabetes. AFABP is believed, but have never been directly proven, to be centrally important in the transport of fatty acids. This study utilizes transgenic mice in which the AFABP gene has been disrupted by homologous recombination and which lack the AFABP protein. Given the abundance and tissue-specificity of AFABP, the absence of the protein should alter adipocyte lipid homeostasis. Using the AFABP-knockout mice, the proposed research will investigate the role of AFABP in fatty acid transport into and within adipocytes, as well as in the regulation of white and brown adipocyte lipid metabolism.

所提出的研究的目标是确定 脂肪细胞脂肪酸结合蛋白（AFABP）在转运和 脂肪组织中脂肪酸的代谢。 脂肪酸贩运是 对脂肪组织储存和调动能量的能力至关重要， 并且脂肪酸代谢控制的不平衡伴随着肥胖， 胰岛素抵抗和糖尿病。 AFABP被认为是，但从来没有 直接证明，在脂肪的运输中起着重要的作用， acids. 这项研究利用转基因小鼠，其中AFABP基因具有 被同源重组破坏，并且缺乏AFABP 蛋白 考虑到AFABP的丰度和组织特异性， 该蛋白质的缺乏将改变脂肪细胞脂质稳态。 使用 AFABP基因敲除小鼠，拟议的研究将调查的作用 AFABP在脂肪酸转运进入脂肪细胞和脂肪细胞内，以及 调节白色和棕色脂肪细胞的脂质代谢。