FUNCTIONAL ANALYSIS OF ADIPOCYTE LIPID BINDING PROTEIN

脂肪细胞脂质结合蛋白的功能分析

• 批准号: 2634173
• 负责人: ELIZABETH R SMITH
• 金额: $ 3.05万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-12-08 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2634173
• 关键词: adipocytes; fatty acid binding protein; fatty acid metabolism; fatty acid transport; obesity

The goal of the proposed research is to determine the function of the adipocyte fatty acid binding protein (AFABP) in the transport and metabolism of fatty acids in adipose tissue. Fatty acid trafficking is central to the ability of adipose tissue to store and mobilize energy, and imbalances in the control of fatty acid metabolism accompany obesity, insulin resistance, and diabetes. AFABP is believed, but have never been directly proven, to be centrally important in the transport of fatty acids. This study utilizes transgenic mice in which the AFABP gene has been disrupted by homologous recombination and which lack the AFABP protein. Given the abundance and tissue-specificity of AFABP, the absence of the protein should alter adipocyte lipid homeostasis. Using the AFABP-knockout mice, the proposed research will investigate the role of AFABP in fatty acid transport into and within adipocytes, as well as in the regulation of white and brown adipocyte lipid metabolism.

拟议研究的目的是确定 运输中的脂肪细胞脂肪酸结合蛋白（AFABP）和 脂肪组织中脂肪酸的代谢。 脂肪酸运输是 脂肪组织能够存储和动员能量的能力的中心， 肥胖伴随着脂肪酸代谢的控制不平衡， 胰岛素抵抗和糖尿病。 相信AFABP，但从未 直接证明，在脂肪的运输中非常重要 酸。 这项研究利用AFABP基因具有的转基因小鼠 受到同源重组的破坏，缺乏AFABP 蛋白质。 鉴于AFABP的丰度和组织特异性 缺乏蛋白质应改变脂肪细胞脂质稳态。 使用 AFABP-KNOCKOUT小鼠，拟议的研究将调查该角色 脂肪酸中的AFABP进入脂肪细胞和内部 在调节白色和棕色脂肪细胞脂质代谢中。