FUNCTIONAL ANALYSIS OF ADIPOCYTE LIPID BINDING PROTEIN

拟议研究的目标是确定 脂肪细胞脂肪酸结合蛋白(AFABP)在转运和 脂肪组织中脂肪酸的代谢。脂肪酸走私是 脂肪组织储存和调动能量的能力至关重要， 而脂肪酸代谢控制的失衡会伴随着肥胖， 胰岛素抵抗和糖尿病。AFABP被认为，但从来都不是 直接证明，在脂肪的运输中起着中心作用 酸。这项研究利用了AFABP基因具有 被同源重组破坏，并且缺乏AFABP 蛋白。鉴于AFABP的丰富性和组织特异性， 蛋白质的缺失会改变脂肪细胞的脂类平衡。vbl.使用 在AFABP基因敲除小鼠中，拟议的研究将调查其作用 AFABP在脂肪细胞内和脂肪细胞内脂肪酸运输中的作用 在调节白色和棕色脂肪细胞的脂肪代谢方面。

The goal of the proposed research is to determine the function of the adipocyte fatty acid binding protein (AFABP) in the transport and metabolism of fatty acids in adipose tissue. Fatty acid trafficking is central to the ability of adipose tissue to store and mobilize energy, and imbalances in the control of fatty acid metabolism accompany obesity, insulin resistance, and diabetes. AFABP is believed, but have never been directly proven, to be centrally important in the transport of fatty acids. This study utilizes transgenic mice in which the AFABP gene has been disrupted by homologous recombination and which lack the AFABP protein. Given the abundance and tissue-specificity of AFABP, the absence of the protein should alter adipocyte lipid homeostasis. Using the AFABP-knockout mice, the proposed research will investigate the role of AFABP in fatty acid transport into and within adipocytes, as well as in the regulation of white and brown adipocyte lipid metabolism.