IMPROVED DETECTION OF LARGE BIOMOLECULES IN TOF-MS

改进 TOF-MS 中大生物分子的检测

• 批准号: 2285156
• 负责人: W B FELLER
• 金额: $ 8.1万
• 依托单位: NOVA SCIENTIFIC, INC.
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-07-01 至 1995-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2285156
• 关键词: biomedical equipment development; fiber optics; hydrogen; ionization; lasers; macromolecule; mass spectrometry; polymers; reduction; semiconduction; surface property

One of the most desired improvements in the study of high mass biopolymers in time-of-flight mass spectrometry is increased detector sensitivity and efficiency. The innovations we propose will substantially improve microchannel plates (MCPs) as detectors of high mass biomolecular ions. This will be accomplished through implementation of three separate developments: (1) reducing the MCP surface work function, to increase ion- electron and ion-ion conversion yields leading to improved signal-to-noise ratios (2) enhancing the grazing incidence geometry for incident primary ions through use of a novel square channel geometry, also resulting in increased ion-electron and ion-ion yields: and (3) substantially improving MCP detector dynamic range, which has recently been a limiting factor in analyzing heavier biomolecules in matrix-assisted laser desorption ionization (MALDI). Any one of these improvements would be considered a worthy incremental enhancement in MCP performance. We propose to implement all three simultaneously in the same MCP detector; the result would be a radical departure in the performance of high mass ion detectors. If this is done, MCP detectors will quickly assume the leading role in high mass ion detection in the 50,000 to 1 MegaDalton mass range--just as they are now well established as the leading detectors for low mass ions.

高质量生物聚合物研究中最令人期待的改进之一 在飞行时间质谱中，增加了检测器灵敏度， 效率我们提出的创新将大大改善 微通道板（MCP）作为高质量生物分子离子的检测器。 这将通过实施三个独立的 发展：（1）降低MCP表面功函数，以增加离子- 电子和离子-离子转换产率导致改善的信噪比 比率（2）增强入射初级的掠入射几何形状 离子通过使用一种新的方形通道几何形状，也导致 增加的离子-电子和离子-离子产率;和（3）显著改善 MCP检测器动态范围，最近已成为限制因素， 在基质辅助激光解吸中分析较重的生物分子 电离（MALDI）。任何一项改进都将被视为 MCP性能的有价值的增量增强。我们建议实施 所有三个同时在同一个MCP检测器;结果将是一个 大质量离子检测器性能的根本偏离。如果这 完成后，MCP探测器将很快在高质量领域发挥主导作用。 在50，000到1兆道尔顿质量范围内的离子检测--就像它们一样 现在已被公认为低质量离子的主要检测器。