RAPID TESTSTRIP ASSAY FOR ACETAMINOPHEN AND SALICYLATE

对乙酰氨基酚和水杨酸盐的快速试纸测定

• 批准号: 3499108
• 负责人: HANS G KLOEPFER
• 金额: $ 8.02万
• 依托单位: MICRONIX, INC.
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-01-01 至 1994-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3499108
• 关键词: acetaminophen; blood chemistry; colorimetry; diagnosis design /evaluation; diagnostic tests; drug detection; method development; noninvasive diagnosis; rapid diagnosis; reagent /indicator; salicylate; saliva; serology /serodiagnosis; urinalysis

Acetaminophen and salicylates are contained in over 200 prescription and non-prescription formulations. Of all intentional and unintentional overdose ingestions of pharmaceutical and non-pharmaceutical compounds, those of acetaminophen and salicylates are the most frequent, both in number of exposures and fatalities. Many exposures are co-intoxications of both drugs. Approximately one half of all patients are children. The need for rapid estimation of acetaminophen and salicylates in acute intoxication and therapeutic monitoring is well documented. Rapid test methods for use by non-laboratory personnel in emergency and point-of- care environments are currently unavailable. Some laboratory spot-tests for urine exist but they lack specificity, and timely specimen acquisition, transfer and analysis can be problematic. Available methods on automated instruments and compact analyzers are scarce and cost- intensive. Subject of this proposal is the development of a novel non- instrumented dual-analyte teststrip method for the rapid simultaneous semi-quantitation of acetaminophen and salicylates from unmeasured micro-specimens of whole blood, saliva and urine. The method is specific for each of the two drugs as enzymic-colorimetric detection principles are employed. Dry reagent technology enhances method stability and product life time. Contributions of the devices are its functionality in a near-patient, non-laboratory environment, early detection, timely institution of treatment, and cost-savings.

对乙酰氨基酚和水杨酸酯包含在200多个处方中， 非处方配方。 所有有意和无意的 药物和非药物化合物的过量摄入 对乙酰氨基酚和水杨酸酯的那些是最常见的 暴露和死亡人数。 许多暴露是共毒性 两种药物。 所有患者中约有一半是儿童。 这 需要快速估算急性中对乙酰氨基酚和水杨酸盐 中毒和治疗监测已得到充分记录。 快速测试 非实验室人员使用的方法 目前不可用的护理环境。 一些实验室现场测试 存在尿液，但缺乏特殊性和及时的标本 获取，转移和分析可能是有问题的。 可用方法 关于自动仪器和紧凑分析仪的稀缺和成本 - 密集的。 该提议的主题是一种新颖的非 - 快速同时进行的仪器双分析物测试条方法 对乙酰氨基酚和水杨酸盐的半定量 全血，唾液和尿液的微特征。 该方法是特定的 对于两种药物，每种药物都作为酶 - 色检测原理 被雇用。 干试剂技术增强了方法稳定性和 产品寿命。 设备的贡献是其功能 在接近病人的非实验室环境中，早期检测，及时 治疗机构和节省成本。