T CELL FUNCTIONAL DIFFERENTIATION IN THE INTESTINE

肠道中 T 细胞的功能分化

• 批准号: 2146393
• 负责人: Terrence A. Barrett
• 金额: $ 14.31万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-08-01 至 1999-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2146393
• 关键词: antigens; apoptosis; cell cycle; cell differentiation; flow cytometry; genetically modified animals; helper T lymphocyte; immunocytochemistry; interferon gamma; interleukin 2; interleukin 4; laboratory mouse; leukocyte activation /transformation; oral administration; ovalbumin; secretory immune system; tissue /cell culture

The goal of these studies will be to understand the role of the intestinal environment on influencing the responses of CD4+ T cells to oral proteins. Mucosal surfaces such as the intestine are exposed to a wide array of exogenous antigen (Ag). The introduction of Ag in these tissues leads to a tightly-regulated cascade of events that result in distinct local and systemic responses. CD4+ T cells play a critical role in coordinating these responses by directing activities of other cell types through the release of specific arrays of lymphokines. A better understanding of how CD4+ T cells respond to oral antigen may have important clinical applications as recent studies have successfully sensitized immune responses to oral carcinogens and enhanced mucosal immunity in diseases such as AIDS. Recent studies in patients with multiple sclerosis and rheumatoid arthritis have shown that oral feeding of tissue proteins may provide a new approach to decreasing disease recurrences in autoimmune diseases. Therefore, we will focus our studies on examining critical aspects of the mucosal T cell response to exogenous antigen. We will utilize a T cell receptor transgenic murine model specific for a peptide of chicken ovalbumin (Ova). By studying intestinal T cells in control and Ova-fed mice we will be able to examine the factors which influence the development of intestinal T cell responses.

这些研究的目的是了解肠道的作用， 环境影响CD4+ T细胞对口服蛋白质的反应。 粘膜表面如肠暴露于各种各样的 外源性抗原（Ag）。在这些组织中引入Ag导致 一系列严格控制的事件，导致不同的局部和 系统性反应。CD4+ T细胞在协调免疫系统中起着关键作用。 这些反应通过引导其他细胞类型的活动， 释放特定的淋巴因子阵列。更好地了解如何 CD4+ T细胞应答口服抗原可能具有重要的临床意义 最近的研究已经成功地使免疫 对口腔致癌物的反应和疾病中增强的粘膜免疫 例如艾滋病。多发性硬化症患者的最新研究， 类风湿性关节炎已经表明，组织蛋白的口服喂养可能 为减少自身免疫性疾病的复发提供了一种新的方法， 疾病因此，我们的研究将集中在审查关键 粘膜T细胞对外源性抗原的应答。我们将 利用对肽特异的T细胞受体转基因鼠模型 鸡卵清蛋白（Ova）通过研究对照组和 我们将能够检查影响卵母细胞生长的因素。 肠道T细胞反应的发展。