DEVELOPMENT OF HEAD NERVES, BLOOD VESSELS, BONES, MUSCLE

头部神经、血管、骨骼、肌肉的发育

• 批准号: 2129365
• 负责人: DREW M NODEN
• 金额: $ 19.58万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-08-01 至 1997-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2129365
• 关键词: angiogenesis; antibody; biomarker; bone development; cell cell interaction; chick embryo; connective tissue development; developmental neurobiology; head; innervation; motor neurons; musculoskeletal system; myoblasts; myogenesis; neural crest; neurogenesis; neuromuscular function; quail; skull; trigeminal nerve

DESCRIPTION (adapted from the Investigator's abstract): The goal of this research is to characterize the cell interactions underlying positional specification of sensory and motor neurons and their targets during development of the avian head. Emphasis will be on studies of the trigeminal- jaw complex. Studies of cell movements and lineages as well as changes in cell behavior resulting from experimental alteration in the spatial relations between precursors and their environment will be performed. The first aim will define interactions leading to the formation of specific connections between trigeminal motor nerves and jaw muscles. Lineage tracing of neuroblasts and myoblasts will be performed. Experimental strategies involve use of retroviral markers, injection of fluorochrome tracers, and surgical transplantation of rhombomeres (motor neuron precursors) and somitomeres (muscle precursors) between quail and chick embryos. The second aim focuses on specification of trigeminal sensory neurons derived from neural crest and placodal cells, and tests the hypothesis that the latter are dependent upon contact with the former for spatial patterning.This will be tested by surgical manipulation of precursors, followed by a detailed analysis of the central and peripheral projections of both graft- and host- derived trigeminal sensory axons using antibodies specific to quail or chick axons. The third aim will examine two features of developing craniofacial skeletal muscles, the origins of primary and secondary myocytes and myocyte degeneration. The proposed studies will determine whether avian primary and secondary myocytes have common or separate embryonic origins. Most primary myocytes in developing head muscles degenerate between days 12-15 in the chick embryo. Studies are planned to study the relationships between innervation and myocyte degeneration during craniofacial muscle development. The possibility that changes in innervation patterns are related to myocyte degeneration will be studied. The final aim examines the fate of single neural crest progenitors to study the strategies used by neural crest cells to generate the diversity of phenotypes found in the head. Clonal analyses of neural crest cells will be performed in vivo with retroviral markers. These investigations are expected to define how neurogenic and myogenic cells move and interact before forming functionally integrated tissues in the developing head.

描述（根据调查员的摘要改编）：目的 研究是为了表征位置的细胞相互作用 感觉和运动神经元的规格及其目标 禽脑的发展。 重点是研究 三轮颌络合物。 细胞运动和谱系的研究 随着通过实验改变导致细胞行为的变化 前体与其环境之间的空间关系将是 执行。 第一个目标将定义互动导致 三叉神经和 下颌肌肉。神经细胞和成肌细胞的谱系追踪将是 执行。实验策略涉及使用逆转录病毒标记， 注射荧光体示踪剂和手术移植 菱形（运动神经元前体）和体体（肌肉 前体））鹌鹑和雏鸡胚胎之间。 第二个目标重点 从神经rest和 位置细胞，并检验了后者依赖的假设 与前者接触以进行空间图案。这将进行测试 通过对前体的手术操纵，然后进行详细分析 移植物和宿主的中央和周围投影 使用特定于鹌鹑或 小鸡轴突。第三个目标将检查开发的两个特征 颅面骨骼肌，原发性和次要的起源 心肌细胞和心肌变性。 拟议的研究将确定 鸟类原发性和次级肌细胞是否具有常见或分开 胚胎起源。发育肌肉的大多数主要肌细胞 在小鸡胚胎中12-15天之间退化。 计划进行研究 研究神经和心肌变性之间的关系 在颅面发育期间。 改变的可能性 在神经上，神经模式与心肌变性有关 研究。 最终目的检查了单个神经rest的命运 祖细胞研究神经Crest细胞使用的策略 产生头部中发现的表型的多样性。 克隆 神经rest细胞的分析将在体内用逆转录病毒进行 标记。 预计这些研究将定义神经源和 肌原细胞在形成功能整合之前移动和相互作用 发育中心的组织。