DEVELOPMENT OF HEAD NERVES, BLOOD VESSELS, BONES, MUSCLE

头部神经、血管、骨骼、肌肉的发育

• 批准号: 3220142
• 负责人: DREW M NODEN
• 金额: $ 17.84万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-08-01 至 1993-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3220142
• 关键词: angiogenesis; cartilage development; chick embryo; coronary vessels; developmental neurobiology; fluorescence microscopy; growth /development; head; immunocytochemistry; mesoderm; myofibrils; myogenesis; neural crest; neurogenesis; quail; skull; trigeminal nerve; vascular endothelium

The objectives of my research are to characterize the movements of all craniofacial mesenchymal cells in the avian embryo, from their origins through the establishment of fully differentiated tissues, and to analyze the interactions that regulate patterned assembly of mesenchymal populations into craniofacial tissues and organs. Data generated during the current funding period revealed the following heretofore unreported features of avian craniofacial development: (1) a series of deep, segmental pathways by which neural crest cells move into paraxial mesoderm, (2) multiple origins and invasive movements of angioblasts, and their dependency upon cues in head mesenchyme for assembly into blood vessels, (3) a mesenchymal population that develops at the endoderm:mesoderm interface shortly before the onset of blood vessel formation but is only visible in SEM preparations, (4) patterns of myotube alignment that appear early in muscle development, are unique in each branchial arch muscle, and presage the subsequent patterns of segregation of individual muscles, and (5) precise details of cell movements and patterns of nerve fiber growth during development of the trigeminal sensory-motor complex. The proposed new experiments will extend analyses of each of these tissues, and focus greater attention on defining the tissue interactions necessary for their coordinated formation. Specific proposed experiments will examine the relative contributions of different angioblast precursors in development of craniofacial blood vessels, particularly those in the hindbrain, face, and heart wall. These will be determined by studying the formation of transplanted quail endothelial precursors, which can be recognized immunocytochemically, developing in chick host embryos. To define myogenic interactions, TEM and fiber type analyses will be performed on muscles developing from transplanted myogenic precursors that normally would express different features. Finally, interactions underlying specification of trigeminal sensory nerves, as evidence by their ability to form specific central and peripheral projections, will be assessed using several fiber-tracing methods on embryos in which the ganglionic precursors (neural crest or placodal tissues) have been transplanted to different locations or at different times.

我的研究的目标是表征所有人的动作 从其起源起源于禽类胚胎中的颅面间充质细胞 通过建立完全分化的组织，并分析 调节间充质组装的相互作用 种群进入颅面组织和器官。 当前资金期间生成的数据显示了以下 迄今未报告的禽类颅面开发特征：（1） 神经rest细胞进入的一系列深层节段性途径 近距离中胚层，（2）多发性和侵入性运动 血管细胞及其对组装头部间充质的提示的依赖性 进入血管，（3）在 内胚层：血管发作前不久中胚层界面 形成，但仅在SEM制剂中可见，（4）Myotube的模式 在肌肉发育早期出现的一致性在每个方面都是独特的 分支拱形肌肉，并预示随后的隔离模式 单个肌肉，以及（5）细胞运动的精确细节和 神经纤维生长的模式在三叉神经的发展过程中 感觉运动复合物。 提出的新实验将扩展对每种组织的分析， 并将更大的注意力集中在定义必要的组织相互作用上 他们的协调形成。 具体提出的实验将 检查不同血管生成前体在中的相对贡献 颅面血管的发展，尤其是那些 后脑，脸和心墙。 这些将通过研究 移植鹌鹑内皮前体的形成，可以是 在雏鸡宿主胚胎中发育的免疫细胞化学识别。 到 将执行定义肌原相互作用，TEM和纤维类型分析 关于从通常的肌源性前体发展的肌肉 将表达不同的功能。 最后，互动的基础 三叉学感觉神经的规范，作为其能力的证据 将使用特定的中央和外围预测。 神经节前体的胚胎上的几种纤维追踪方法 （神经rest或placodal组织）已移植到不同 位置或不同时间。