Interaction Affecting Craniofacial Muscle Differentiatio

影响颅面肌分化的相互作用

• 批准号: 6623471
• 负责人: DREW M NODEN
• 金额: $ 27.65万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6623471
• 关键词: apoptosis; biomarker; cell cell interaction; cell differentiation; chick embryo; connective tissue development; craniofacial; electron microscopy; embryo /fetus cell /tissue; embryo /fetus tissue transplantation; embryogenesis; extraocular muscle; facial muscles; histochemistry /cytochemistry; mesenchyme; mesoderm; microsurgery; myoblasts; myogenesis; myosins; myotubes; neural crest; nonmammalian vertebrate embryology; quail; regulatory gene; rhombencephalon

DESCRIPTION (provided by applicant): Craniofacial muscles show great structural and functional diversity, and anomalies in their development contribute to a wide range of problems in vision, facial expression, mastication, and phonation. However, little is known about processes controlling the differentiation and maturation of branchial and extra-ocular muscles. Head muscles arise in mesenchyme adjacent to the brain then migrate and differentiate in connective tissues derived from the neural crest. Trunk and limb muscles arise in epithelial myotomes, then differentiate in paraxial and lateral mesoderm. This research will characterize tissue interactions necessary (1) to promote early differentiation of branchial and extra-ocular muscle myoblasts and (2) to direct expression of fiber typespecific myosins in primary myotubes in these muscles. These interactions will be identified by transplanting specific muscle precursors, at different stages and with or without putative muscle-inducing tissues, between head and trunk axial levels. Assays of tissue response include expression of myoblast markers (e.g., my, about, myoD), of trunk or head muscle-specific markers (e.g., parc aboutcis, pax3; podl, barx2, Ibxl,en2), and at later stages of muscle-specific myosin heavy chains. Surgeries are done on quail and chick embryos, which are ideally suited for in vivo tissue recombination experiments and for which detailed cellular and molecular biographies of head muscles are uniquely available. A third specific aim will characterize the processes by which craniofacial muscles change their attachments during later growth and maturation stages. EM studies reveal that primary myotubes undergo focal degeneration at their myotendinous tips and also at mid-myotube regions during embryonic days 12-15. I propose that these focal degenerations are essential for normal remodeling of head muscles, but nothing is known of the processes or provocations for these focal losses. Assays for elements of the pathways known to be active during programmed cell death of mononucleated cells will be applied to normal and function- or growth-arrested head muscles. Collectively, these experiments will provide the first insights into the signals necessary for initiation and diverse differentiations of craniofacial muscles and the mechanisms by which remodeling of these head muscles occurs.

描述（由申请人提供）：颅面肌肉显示出很棒的结构 和功能多样性以及其发展中的异常情况有助于 视觉，面部表情，咀嚼和 发声。但是，关于控制该过程的过程知之甚少 分支和眼外肌肉的分化和成熟。头 肌肉发生在大脑附近的间充质中，然后迁移，然后 在衍生自神经rest的结缔组织中分化。树干和 肢体肌肉出现在上皮肌肉肌中，然后在近亲和 中胚层。这项研究将表征必要的组织相互作用 （1）促进分支和眼外肌肉的早期分化 肌细胞和（2）直接表达纤维在原发中的肌球蛋白 这些肌肉中的肌管。这些互动将由 在不同的阶段和OR上移植特定的肌肉前体 没有假定的肌肉诱导组织，在头部和躯干轴向之间。 组织反应的测定包括表达肌细胞标记物（例如，我的， 关于躯干或肌肉特异性标记的Myod） pax3; podl，barx2，ibxl，en2），在肌肉特异性肌球蛋白的后期 重链。手术是在鹌鹑和小鸡胚胎上进行的，理想情况下是 适用于体内组织重组实验，并为此详细 头部肌肉的细胞和分子传记是独特的。一个 第三个特定目的将表征颅面的过程 在以后的生长和成熟阶段，肌肉会改变其依恋。 Em 研究表明，主要的肌管会在其处发生局灶性变性 在胚胎时期12-15期间，肌齿状尖端以及肌管中部区域。 我建议这些焦点变性对于正常重塑至关重要 头部肌肉，但对这些过程或挑衅没有任何了解 焦点损失。在已知在 单核细胞的程序性细胞死亡将应用于正常和 功能或生长障碍的头部肌肉。总的来说，这些实验将 提供对启动必要的信号的首次见解和 颅面肌肉的各种差异和机制 这些头部肌肉的重塑发生。