SPECIFIC REPRESSION OF PROLACTIN GENE EXPRESSION

催乳素基因表达的特异性抑制

• 批准号: 2143169
• 负责人: RICHARD N DAY
• 金额: $ 12.08万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-07-11 至 1998-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2143169
• 关键词: DNA footprinting; RNA binding protein; RNA splicing; estradiol; estrogen receptors; gel mobility shift assay; genetic promoter element; genetic regulatory element; hormone regulation /control mechanism; immunoprecipitation; laboratory rat; neoplasm /cancer transplantation; northern blottings; nuclear runoff assay; prolactin; repression; site directed mutagenesis; solution hybridization; somatotropin; tissue /cell culture; transcription factor; transfection; western blottings

DESCRIPTION (adapted from the applicant's abstract): The objective of the proposed studies is to identify the critical molecular events that direct specific repression of PRL gene expression. Aim 1 is to establish the importance of the alternative splice form of Pit-1 in the repression of PRL and to determine the molecular events that contribute to the splice choice. Aim 2 is to determine if specific DNA elements in the PRL gene are the key determinants of why PRL gene expression is inhibited in GH3 cell-induced tumors, while GH gene expression is not. Aim 3 will be to examine the interaction of proteins with the PRL and GH promoters, and to characterize the proteins that are involved in the specific repression of the PRL gene.

描述(改编自申请人的摘要)：目标 拟议的研究是为了确定关键的分子事件， 直接特异性抑制PRL基因表达。目标1是 确定Pit-1的另一种剪接形式在 抑制PRL并确定与之相关的分子事件 到拼接选择。目标2是确定特定的DNA元素是否存在于 PRL基因是PRL基因表达的关键决定因素 在GH3细胞诱导的肿瘤中被抑制，而GH基因的表达不受抑制。 目标3将检查蛋白质与PRL和PRL之间的相互作用 GH启动子，并鉴定参与GH启动子的蛋白质。 PRL基因的特异性抑制。