SPECIFIC REPRESSION OF PROLACTIN GENE EXPRESSION

催乳素基因表达的特异性抑制

• 批准号: 6517210
• 负责人: RICHARD N DAY
• 金额: $ 19.9万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-07-11 至 2003-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6517210
• 关键词: biological signal transduction; cell cycle; cell nucleus; estrogen receptors; fluorescence microscopy; fluorescence resonance energy transfer; gel mobility shift assay; gene induction /repression; genetic regulatory element; genetic transcription; green fluorescent proteins; hormone regulation /control mechanism; immunofluorescence technique; immunoprecipitation; mitogen activated protein kinase; nuclear matrix; polymerase chain reaction; prolactin; protein localization; protein protein interaction; protein sequence; protein structure function; reporter genes; site directed mutagenesis; transcription factor

DESCRIPTION: (Adapted from applicant's abstract) The broad objective of this proposal will be to continue the ongoing studies to map protein protein interactions of Pit1. This study will begin to assess how cell signaling influences such interactions and, specifically, it is proposed to assess how an Ets repressor protein ERF negatively regulates prolactin gene regulation. First, the applicant proposes to identify the endogenous ERF protein and characterize its subcellular localization during critical periods within the cell cycle and in response to activation of signal transduction cascades. Second, the requirements for Pit-1 nuclear localization will be precisely identified so as to permit further protein protein analysis by FRET microscopy. Continuing on this theme, the final aim will investigate the association of Pit-1 and its protein partners with the nuclear matrix.

描述：（改编自申请人的摘要） 建议将继续正在进行的研究，以绘制蛋白质 pit 1的互动这项研究将开始评估细胞信号是如何 影响这种相互作用，特别是，建议评估如何 Ets阻遏蛋白ERF负性调节催乳素基因调控。 首先，申请人提出鉴定内源性ERF蛋白， 表征其亚细胞定位在关键时期内， 细胞周期和响应信号转导级联的激活。 第二，Pit-1核国产化的要求， 鉴定，以便允许通过FRET显微镜进行进一步的蛋白质分析。 继续这一主题，最后的目标将调查协会的 Pit-1及其蛋白质与核基质结合。