SPECIFIC REPRESSION OF PROLACTIN GENE EXPRESSION

催乳素基因表达的特异性抑制

• 批准号: 2855300
• 负责人: RICHARD N DAY
• 金额: $ 18.67万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-07-11 至 2003-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2855300
• 关键词: biological signal transduction; cell cycle; cell nucleus; estrogen receptors; fluorescence microscopy; fluorescence resonance energy transfer; gel mobility shift assay; gene induction /repression; genetic regulatory element; genetic transcription; green fluorescent proteins; hormone regulation /control mechanism; immunofluorescence technique; immunoprecipitation; mitogen activated protein kinase; nuclear matrix; polymerase chain reaction; prolactin; protein localization; protein protein interaction; protein sequence; protein structure function; reporter genes; site directed mutagenesis; transcription factor

DESCRIPTION: (Adapted from applicant's abstract) The broad objective of this proposal will be to continue the ongoing studies to map protein protein interactions of Pit1. This study will begin to assess how cell signaling influences such interactions and, specifically, it is proposed to assess how an Ets repressor protein ERF negatively regulates prolactin gene regulation. First, the applicant proposes to identify the endogenous ERF protein and characterize its subcellular localization during critical periods within the cell cycle and in response to activation of signal transduction cascades. Second, the requirements for Pit-1 nuclear localization will be precisely identified so as to permit further protein protein analysis by FRET microscopy. Continuing on this theme, the final aim will investigate the association of Pit-1 and its protein partners with the nuclear matrix.

描述：(改编自申请者的摘要)这项工作的总体目标 建议将继续正在进行的蛋白质图谱研究 PIT1的相互作用。这项研究将开始评估细胞信号如何 影响这种交互，具体地说，建议评估 Ets抑制蛋白ERF负性调控催乳素基因。 首先，申请人提议鉴定内源性ERF蛋白和 表征其在关键时期内的亚细胞定位 细胞周期和对信号转导激活的反应级联反应。 第二，对Pit-1核定位的要求将恰恰是 进行鉴定，以便通过FRET显微镜进行进一步的蛋白质分析。 继续这一主题，最终目标将调查 PIT-1和它的蛋白质与核基质配对。