IDENTIFICATION OF NIDDM GENES IN THE PIMA INDIAN TRIBE

非胰岛素依赖型糖尿病(NIDDM；2型糖尿病)的遗传学 将在皮马印第安人部落进行研究。这项合作研究将 包括分子遗传学家、流行病学家和 临床医生致力于寻找导致NIDDM的遗传因素。 大量的多胎家庭和同胞配对已经被鉴定出来 并进行了DNA分型和分析 已经开始了。最初的努力将集中在人类的四个领域 使用同胞对显示与NIDDM连锁的部分证据的染色体 分析。此外，还将对众多候选基因进行调查 并将继续对基因组进行系统调查，使用高度 微卫星重复序列多态与限制性片段 长度多态。数据将使用多个不同的 连锁分析方法，包括同胞对分析，常规 连锁分析，以及对性状的量化测量，如 肥胖、糖耐量和胰岛素抵抗。连锁检测 之后将进行详细的分子遗传分析，以确定 NIDDM易感性的基础。联系也将在其他 人口和种族群体。

The genetics of non-insulin dependent diabetes (NIDDM; Type 2 diabetes) will be studied in the Pima Indian Tribe. This collaborative study will include the efforts of molecular geneticists, epidemiologists and clinicians focused on finding the inherited factors which lead to NIDDM. A large number of multiplex families and sib pairs have been identified and collected and some DNA typing and analysis has been carried out already. Initially efforts will be focused on 4 regions of human chromosomes which show some evidence for linkage to NIDDM using sib-pair analysis. In addition, numerous candidate genes will also be surveyed and a systematic survey of the genome will be continued using highly polymorphic microsatellite repeat polymorphisms and restriction fragment length polymorphisms. Data will be analyzed using a number of different linkage analysis approaches including sib-pair analysis, conventional linkage analysis, and also quantitative measures of traits such as obesity, glucose tolerance and insulin resistance. Detection of linkage will be followed by detailed molecular genetic analysis to determine the basis of NIDDM susceptibility. Linkages will also be tested in other populations and ethnic groups.