IDENTIFICATION OF NIDDM GENES IN THE PIMA INDIAN TRIBE

皮马印第安部落 NIDDM 基因的鉴定

• 批准号: 2147120
• 负责人: DONALD W BOWDEN
• 金额: $ 24.08万
• 依托单位: WAKE FOREST UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-09-30 至 1998-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2147120
• 关键词: Native Americans; diabetes mellitus genetics; family genetics; genetic markers; genetic polymorphism; glucose tolerance test; human genetic material tag; insulin sensitivity /resistance; linkage mapping; molecular cloning; noninsulin dependent diabetes mellitus; obesity

The genetics of non-insulin dependent diabetes (NIDDM; Type 2 diabetes) will be studied in the Pima Indian Tribe. This collaborative study will include the efforts of molecular geneticists, epidemiologists and clinicians focused on finding the inherited factors which lead to NIDDM. A large number of multiplex families and sib pairs have been identified and collected and some DNA typing and analysis has been carried out already. Initially efforts will be focused on 4 regions of human chromosomes which show some evidence for linkage to NIDDM using sib-pair analysis. In addition, numerous candidate genes will also be surveyed and a systematic survey of the genome will be continued using highly polymorphic microsatellite repeat polymorphisms and restriction fragment length polymorphisms. Data will be analyzed using a number of different linkage analysis approaches including sib-pair analysis, conventional linkage analysis, and also quantitative measures of traits such as obesity, glucose tolerance and insulin resistance. Detection of linkage will be followed by detailed molecular genetic analysis to determine the basis of NIDDM susceptibility. Linkages will also be tested in other populations and ethnic groups.

非胰岛素依赖型糖尿病（NIDDM；2 型糖尿病）的遗传学 将在皮马印第安部落进行研究。 这项合作研究将 包括分子遗传学家、流行病学家和 临床医生专注于寻找导致 NIDDM 的遗传因素。 已鉴定出大量多重家族和同胞对 并收集并进行了一些 DNA 分型和分析 已经。 最初的努力将集中在人类的 4 个区域 使用同胞对显示与 NIDDM 连锁的一些证据的染色体 分析。 此外，还将调查众多候选基因 并将继续使用高度的基因组系统调查 多态性微卫星重复多态性和限制性片段 长度多态性。 将使用多种不同的方法对数据进行分析 连锁分析方法，包括同胞对分析、传统方法 连锁分析，以及特征的定量测量，例如 肥胖、葡萄糖耐量和胰岛素抵抗。 联动检测 随后将进行详细的分子遗传分析以确定 NIDDM 易感性的基础。 链接也将在其他方面进行测试 人口和种族群体。