MECHANISMS OF VISUAL EXCITATION

视觉兴奋机制

• 批准号: 2158925
• 负责人: ALAN FEIN
• 金额: $ 18.9万
• 依托单位: UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-01 至 1998-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2158925
• 关键词: Urodela; alternatives to animals in research; calcium; cell membrane; cellular respiration; electrophysiology; endoplasmic reticulum; fluorescent dye /probe; fresh water environment; inositol phosphates; light adaptations; membrane proteins; photochemistry; retinal adaptation; saltwater environment; visual photoreceptor; visual phototransduction; visual stimulus

DESCRIPTION: The applicant proposes experiments along several lines, all ultimately directed towards a better understanding of the cell biology of photoreceptor cells, both in vertebrates and invertebrates. In vertebrate photoreceptors, three specific aims will be pursued: (1) to further understand the mechanisms of bleaching adaptation, the longitudinal spread of the effects of bleaching desensitization will be determined and the effects of 11-cis-locked analogs and light polarization on these phenomena will be explored; (2) to understand the mechanisms that may account for limited spread between inner and outer segment of integral plasma membrane proteins, the lateral diffusion of lipid soluble fluorescent probes restricted to the plasma membrane will be quantitated; and (3) to understand the control of respiration in photoreceptor mitochondria, the O2 consumption in isolated rods will be measured and the regulation of this consumption by light and cytoplasmic molecules such as Ca, ATP and ADP will be examined. In studies of invertebrate photoreceptors, under aim 4, the effects of IP5 on light- sensitive voltages and the control of Ca cytoplasmic concentration will be determined.

产品说明： 申请人提出了沿着几条线的实验， 所有这些最终都是为了更好地理解细胞 感光细胞生物学，包括脊椎动物和无脊椎动物。 在脊椎动物光感受器中，将追求三个具体目标：（1） 为了进一步了解漂白适应的机制， 漂白脱敏效果的纵向传播将是 确定和11-顺式锁定类似物和光的影响 对这些现象的两极分化将进行探讨;（2）了解 可以解释内部和外部之间有限传播的机制 完整的质膜蛋白片段，横向扩散， 限制于质膜的脂溶性荧光探针将 （3）了解呼吸的控制， 光感受器线粒体，O2消耗在孤立的杆将是 测量和调节这种消耗的光和细胞质 将检测Ca、ATP和ADP等分子。 研究中 无脊椎动物光感受器，根据目标4，IP 5对光的影响- 敏感的电压和Ca胞质浓度的控制将 被确定。