CALCIUM REGULATION IN MEGAKARYOCYTES, THE PROGENITOR OF PLATELETS

该项目的重点是已知的振荡现象， [Ca2+]i。 已知这些振荡是 在细胞内cGMP水平增加时可逆性抑制，或 营 此外，电生理学证据表明， 巨核细胞中钙振荡激活的机制， 血小板与许多其他类型的细胞不同， 这些细胞不表达兰尼碱或咖啡因敏感的钙库。 该建议是利用国家的成像能力， 资源，以进一步的实验解剖的钙 振荡，并使用虚拟细胞来模拟这一过程， 似乎比其他钙功能更复杂 振荡器 私家侦探认为，几何学，简单的药理学 巨核细胞单一的Ca ~（2+）释放系统使其具有良好的生物学特性 虚拟细胞研究的候选人。 研究科同意 in this view视图. 这个项目非常适合拟议的用途， 国家稀土源，而且，由于其重点是钙动力学，也 将受益于钙动力学的核心项目。 研究科 对这个项目持积极态度。

This project focuses on the known phenomenon of oscillations of [Ca2+]i in rat megakaryocytes. These oscillations are known to be inhibited reversibly upon increase of intracellular levels of cGMP or cAMP. Further, electrophysiological evidence suggests that the mechanism of activation of Ca oscillations in megakaryocytes and platelets is different from that in many other cell types in that these cells do not express ryanodine- or caffeine-sensitive Ca stores. The proposal is to use the imaging capabilities of the National Resource to further the experimental dissection of the calcium oscillations and to use the Virtual Cell to model this process, which appears to function with less complexity than other calcium oscillators. The PI suggests that the geometry, simple pharmacology and single Ca2+ release system of megakaryocytes makes them good candidates for study by the Virtual Cell. The Study Section concurs in this view. This project fits well with the proposed use of the National Re source, and, as its focus is an calcium dynamics, also will benefit from the core project on Ca dynamics. The Study Section is positive toward this project.