CALCIUM REGULATION IN MEGAKARYOCYTES, THE PROGENITOR OF PLATELETS

这个项目的重点是已知的振荡现象 [Ca2]i在大鼠巨核细胞中表达。这些振荡是已知的 在细胞内cGMP或cGMP水平升高时可逆抑制 夏令营。此外，电生理学证据表明， 巨核细胞内钙振荡的激活机制及机制 血小板与许多其他细胞类型的不同之处在于 这些细胞不表达兰尼定或咖啡因敏感的钙库。 该提议是利用国家航空航天局的成像能力 进一步进行钙离子实验解剖的资源 并使用虚拟细胞来模拟这一过程，这是 似乎比其他钙的作用更简单 振荡器。PI建议几何学，简单的药理学 巨核细胞单一的钙释放系统使其良好 参加虚拟细胞研究的候选人。研究组对此表示赞同 在这个视图中。此项目与建议使用的 国家稀土来源，而且，由于其重点是钙动力学，还 将受益于钙动力学核心项目。研究组 对这个项目持积极态度。

This project focuses on the known phenomenon of oscillations of [Ca2+]i in rat megakaryocytes. These oscillations are known to be inhibited reversibly upon increase of intracellular levels of cGMP or cAMP. Further, electrophysiological evidence suggests that the mechanism of activation of Ca oscillations in megakaryocytes and platelets is different from that in many other cell types in that these cells do not express ryanodine- or caffeine-sensitive Ca stores. The proposal is to use the imaging capabilities of the National Resource to further the experimental dissection of the calcium oscillations and to use the Virtual Cell to model this process, which appears to function with less complexity than other calcium oscillators. The PI suggests that the geometry, simple pharmacology and single Ca2+ release system of megakaryocytes makes them good candidates for study by the Virtual Cell. The Study Section concurs in this view. This project fits well with the proposed use of the National Re source, and, as its focus is an calcium dynamics, also will benefit from the core project on Ca dynamics. The Study Section is positive toward this project.