MECHANISMS OF VISUAL EXCITATION

视觉兴奋机制

• 批准号: 2158926
• 负责人: ALAN FEIN
• 金额: $ 19.02万
• 依托单位: UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-01 至 1998-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2158926
• 关键词: Urodela; alternatives to animals in research; calcium; cell membrane; cellular respiration; electrophysiology; endoplasmic reticulum; fluorescent dye /probe; fresh water environment; inositol phosphates; light adaptations; membrane proteins; photochemistry; retinal adaptation; saltwater environment; visual photoreceptor; visual phototransduction; visual stimulus

DESCRIPTION: The applicant proposes experiments along several lines, all ultimately directed towards a better understanding of the cell biology of photoreceptor cells, both in vertebrates and invertebrates. In vertebrate photoreceptors, three specific aims will be pursued: (1) to further understand the mechanisms of bleaching adaptation, the longitudinal spread of the effects of bleaching desensitization will be determined and the effects of 11-cis-locked analogs and light polarization on these phenomena will be explored; (2) to understand the mechanisms that may account for limited spread between inner and outer segment of integral plasma membrane proteins, the lateral diffusion of lipid soluble fluorescent probes restricted to the plasma membrane will be quantitated; and (3) to understand the control of respiration in photoreceptor mitochondria, the O2 consumption in isolated rods will be measured and the regulation of this consumption by light and cytoplasmic molecules such as Ca, ATP and ADP will be examined. In studies of invertebrate photoreceptors, under aim 4, the effects of IP5 on light- sensitive voltages and the control of Ca cytoplasmic concentration will be determined.

描述：申请人提出了几个方向的实验， 所有这些最终都是为了更好地了解细胞 脊椎动物和无脊椎动物的感光细胞生物学。 在脊椎动物的光感受器中，将追求三个特定的目标：(1) 为了进一步了解漂白适应的机制， 漂白脱感效果的纵向扩散将 11-顺式锁定类似物和光的测定和影响 关于这些现象的极化将被探究；(2)理解 可以解释内部和外部之间有限扩散的机制 一段完整的质膜蛋白，横向扩散 限制在质膜上的脂溶荧光探针 被量化；以及(3)了解儿童呼吸的控制 光感受器线粒体，离体杆的耗氧量将 光和细胞质对这种消耗的测量和调节 将检测钙、三磷酸腺苷和二磷酸腺苷等分子。在…的研究中 无脊椎动物光感受器，在目标4下，IP5对光- 敏感电压与细胞内钙离子浓度的控制 要下定决心。