CELLULAR AND MOLECULAR PHYSIOLOGY OF OCULAR EPITHELIA

描述(改编自申请人的摘要)：液体的分泌 睫状体需要跨上皮运输溶质， 渗水后的水。这个项目中提出的实验 将研究控制离子运动的离子电导 穿过细胞膜，从而控制体液的分泌 睫状体。主要的焦点是确定神经递质是如何 荷尔蒙影响这些离子电导，这可能有助于解释 这些药物对眼压的调节。细胞内 连接神经递质或激素受体的信号通路 睫状体上皮细胞的离子通道也将 调查过了。因为氯离子的传输是一个重要的组成部分 跨上皮液运输，这是该提案的一个方面 重点介绍氯离子通道及其调节。离子机制 将在无色素性和有色素性疾病中进行研究 上皮细胞层。总体目标是建立蜂窝网络 睫状体中上皮转运的有序机制 对眼压控制有更深入的了解 以及可能提供重要靶点的信号通路 可以减少房水流入的药物。

DESCRIPTION (Adapted from applicant's abstract): The secretion of fluid by the ciliary body requires transepithelial transport of solutes, with water following osmotically. The experiments proposed in this project will examine the ionic conductances that control the movement of ions across the cell membrane, and thus control the secretion of fluid by the ciliary body. The principal focus is to determine how neurotransmitters and hormones affect these ionic conductances which may help explain the regulation of intraocular pressure by these agents. The intracellular signaling pathways that link neurotransmitter or hormone receptors to the ion channels of the ciliary epithelial cells will also be investigated. Because transport of chloride ions is a vital component of the transepithelial fluid transport, one aspect of the proposal focuses on chloride channels and their regulation. The ionic mechanisms will be investigated in both the nonpigmented and the pigmented epithelial cell layers. The overall goal is to establish the cellular mechanisms governing epithelial transport in the ciliary body in order to gain a greater understanding of the control of intraocular pressure and of the signaling pathways which may provide important targets for drugs that can reduce aqueous inflow.