CELLULAR AND MOLECULAR PHYSIOLOGY OF OCULAR EPITHELIA

眼上皮的细胞和分子生理学

• 批准号: 2162407
• 负责人: GARY G MATTHEWS
• 金额: $ 22.2万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-09-01 至 1999-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2162407
• 关键词: biological signal transduction; cell membrane; chloride channels; hormone regulation /control mechanism; intraocular aqueous flow; intraocular pressure; ion transport; laboratory rabbit; membrane channels; membrane permeability; neurotransmitters; retinal pigment epithelium; single cell analysis; tissue /cell culture; uvea ciliary body; visual photoreceptor; voltage /patch clamp

DESCRIPTION (Adapted from applicant's abstract): The secretion of fluid by the ciliary body requires transepithelial transport of solutes, with water following osmotically. The experiments proposed in this project will examine the ionic conductances that control the movement of ions across the cell membrane, and thus control the secretion of fluid by the ciliary body. The principal focus is to determine how neurotransmitters and hormones affect these ionic conductances which may help explain the regulation of intraocular pressure by these agents. The intracellular signaling pathways that link neurotransmitter or hormone receptors to the ion channels of the ciliary epithelial cells will also be investigated. Because transport of chloride ions is a vital component of the transepithelial fluid transport, one aspect of the proposal focuses on chloride channels and their regulation. The ionic mechanisms will be investigated in both the nonpigmented and the pigmented epithelial cell layers. The overall goal is to establish the cellular mechanisms governing epithelial transport in the ciliary body in order to gain a greater understanding of the control of intraocular pressure and of the signaling pathways which may provide important targets for drugs that can reduce aqueous inflow.

描述（改编自申请人摘要）：液体分泌 通过睫状体需要溶质的跨上皮转运， 水在后面跟着。本项目提出的实验 将研究控制离子运动的离子电导 通过细胞膜，从而控制液体的分泌， 睫状体主要的焦点是确定神经递质 激素会影响这些离子电导，这可能有助于解释 通过这些药剂调节眼内压。细胞内 连接神经递质或激素受体的信号通路， 睫状上皮细胞的离子通道也将被 研究了因为氯离子的运输是 的跨上皮液体运输，该提案的一个方面， 重点是氯离子通道及其调节。离子机制 将在非色素和色素 上皮细胞层。总的目标是建立细胞 控制睫状体中上皮转运的机制， 为了更好地了解眼内压的控制， 和信号通路，这可能提供重要的目标， 可以减少房水流入的药物。