BIOMEDICAL SEPARATIONS--FIELD-FLOW FRACTIONATION

生物医学分离——场流分离

• 批准号: 2168525
• 负责人: J C GIDDINGS
• 金额: $ 12.83万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-02-01 至 1996-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2168525
• 关键词: analytical method; biomedical equipment development; chromatography; colloids; fluid flow; fluidity; mathematical model; method development; physical separation; sedimentation

The long term objective of the proposed work is to further develop and exploit the potential of field-flow fractionation (FFF) as a tool of wide applicability in the biological sciences. The specific development work aimed at this goal will consist mainly of basic theoretical and experimental studies of FFF performance and optimization, the launching of a program to exploit the multidimensional capabilities of FFF, and more limited projects in applications and basic theory. Work will be undertaken to improve performance through the optimization of channel geometry and dimensions, the further development of split inlet and outlet systems, the optimization of hydrodynamic relaxation, and the fuller utilization of digital methods for data interpretation and instrument control. Programmed FFF will be further optimized. A multidimensional FFF system will be developed by linking together sedimentation FFF and flow FFF systems with the objective of more fully characterizing complex colloids such as those of biological origin. Some applications will be undertaken and further work will be done on broader theoretical and methodological issues in analytical separations.

拟议工作的长期目标是进一步发展和 开发场流分馏(FFF)作为一种工具的潜力 在生物科学领域具有广泛的适用性。具体的发展 以实现这一目标为目标的工作将主要包括基础理论和 前置式火箭发动机性能及优化实验研究 开发FFF的多维功能的程序，以及 在应用和基础理论方面更有限的项目。工作将是 致力于通过优化渠道来提高绩效 几何尺寸、分流进水口的进一步发展和 出水系统，流体动力松弛的优化，以及 充分利用数字方法进行数据解释和 仪器控制。程控FFF将进一步优化。一个 多维FFF系统将通过相互链接来开发 沉淀FFF和流动FFF系统的目标是更充分地 具有复杂胶体特征的，如生物来源的胶体。 将进行一些申请，并将就以下问题开展进一步工作 分析分离中更广泛的理论和方法问题。