BIOMEDICAL SEPARATIONS: FIELD-FLOW FRACTIONATION

生物医学分离：场流分离

• 批准号: 3268165
• 负责人: J C GIDDINGS
• 金额: $ 12.33万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-02-01 至 1996-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3268165
• 关键词: analytical method; biomedical equipment development; chromatography; colloids; fluid flow; fluidity; mathematical model; method development; physical separation; sedimentation

The long term objective of the proposed work is to further develop and exploit the potential of field-flow fractionation (FFF) as a tool of wide applicability in the biological sciences. The specific development work aimed at this goal will consist mainly of basic theoretical and experimental studies of FFF performance and optimization, the launching of a program to exploit the multidimensional capabilities of FFF, and more limited projects in applications and basic theory. Work will be undertaken to improve performance through the optimization of channel geometry and dimensions, the further development of split inlet and outlet systems, the optimization of hydrodynamic relaxation, and the fuller utilization of digital methods for data interpretation and instrument control. Programmed FFF will be further optimized. A multidimensional FFF system will be developed by linking together sedimentation FFF and flow FFF systems with the objective of more fully characterizing complex colloids such as those of biological origin. Some applications will be undertaken and further work will be done on broader theoretical and methodological issues in analytical separations.

拟议工作的长期目标是进一步发展和 发挥场流分级 (FFF) 作为工具的潜力 在生物科学领域具有广泛的应用前景。 具体发展 为实现这一目标而开展的工作主要包括基础理论和 FFF性能和优化的实验研究，推出 开发 FFF 多维能力的程序，以及 应用和基础理论方面的项目比较有限。 工作将是 致力于通过渠道优化提高绩效 几何形状和尺寸，分流入口的进一步发展和 出口系统、流体动力学松弛的优化以及 更充分地利用数字方法进行数据解释和 仪器控制。 编程的FFF将进一步优化。 一个 多维FFF系统将通过链接在一起开发 沉淀 FFF 和流动 FFF 系统的目的是更充分地 表征复杂的胶体，例如生物来源的胶体。 将进行一些申请并开展进一步的工作 分析分离中更广泛的理论和方法论问题。