BIOMEDICAL SEPARATIONS--FIELD-FLOW FRACTIONATION

这项研究计划的长期目标是发展和扩大 场流分馏(FFF)技术，其固有的 优势易于实现，并可应用于复杂的生化 材料。另一个目标是将分离的边界推后 方法论。 要实现上述目标，需要开展四个方面的工作： 1.FFF的总体优化。在这里，我们将寻求改进 仪器、通道设计和程序，以使FFF更多 对广大科学界来说，这是一种可及的、更有用的方法。作为以下内容的一部分 本研究中，我们将考察程控FFF的优化问题。 2.生物化学应用。我们将开展几个直接投资项目 生化相关性，主要与来自 生物医学社区。 3.二维FFF。我们将进行一项重大的长期举措，以 结合了高分辨率和多功能性的二维 FFF在分离中显示出的优势的方法学 大分子和微粒成分。我们希望通过这种方式来进化 新的强大的分离技术。 4.分离科学的发展。在这里，我们将继续我们的 长期努力广泛推进对 基本的分离过程。此外，我们还将寻求开发新的 分离方法和技术。

The long term objective of this research proposal is to develop and expand field-flow fractionation (FFF) technology in such a way that its intrinsic advantages can be readily realized and applied to complex biochemical materials. Another objective is to push back the frontiers of separation methodology. The above objectives are to be met by pursuing work in four categories: 1. General Optimization of FFF. Here we weill seek improvements in instrumentation, channel design, and procedures in order to make FFF more accessible and more useful to the broad scientific community. As part of this study, we will examine the optimization of programmed FFF. 2. Biochemical Applications. We will engage in several projects of direct biochemical relevance, working mainly with collaborators from the biomedical community. 3. Two-Dimensional FFF. We will undertake a major long-term initiative to combine the high resolving power and versatility of two-dimensional methodology with the advantage displayed by FFF in the separation of macromolecular and particulate components. In this way we hope to evolve new and powerful separation techniques. 4. Developments in Separation Science. Here we will continue our long-standing efforts to broadly advance the theoretical understanding of basic separation processes. In addition, we will seek to develop new separation approaches and techniques.