PHOTOAFFINITY LABELS BY LASER SPECTROSCOPY

这项工作的主要目标是提供必要的知识 用于光标记实验的声音应用和解释。一个 生命系统研究的主要目标是联系功能 具有化学结构。已经有几种实验性的方法 为实现这一目标而开发的。其中最有用的是 光标记程序。在这种方法中，可活化试剂是 与目标结构相邻的位置。这通常是实现的 通过利用目标对特定目标的高亲和力 底物。贴标签过程中的关键步骤包括激活 试剂在光照和光照条件下的潜在反应性 试剂与靶生物之间形成共价键 结构。一旦完成这一点，目标结构就可以 分解成更小的部分，并找到链接的标签。该计划的目的是 这个应用程序的研究是为了检查激活的细节 光标记剂，研究共价键的形成过程 形成，开发用作标签的新的光化学物质，并对其进行测试 在实际生物系统中的发现。许多方法将是 被用来实现这些目标的。然而，我们的核心是 方法是时间分辨激光瞬时吸收的应用 光谱学来解决这个问题。这种方法允许直接观察 ，并提供了有关 关键的激活和绑定步骤。激光光谱结果将会 与其他更经典的实验结果相结合 达到本研究的目的。

The major objective of this work is to provide the knowledge necessary for sound application and interpretation of photolabeling experiments. A major objective in the study of living systems is to associate function with chemical structure. Several experimental approaches have been developed to facilitate this goal. Among the most useful of these are photolabeling procedures. In this approach an activatable reagent is positioned adjacent to a target structure. This is often accomplished by taking advantage of the high affinity of the target for a particular substrate. The key steps in the labeling process involve activation of the latent reactivity of the reagent by irradiation with light and covalent bond formation between the reagent and the target biological structure. Once this has been accomplished, the target structure can be broken into smaller parts and the linked label located. The aim of the research in this application is to examine the details of activation of the photolabeling agent by light, study the process of covalent bond formation, develop new photochemicals useful as labels, and test these findings in actual biological systems. A number of methods will be employed to accomplish these goals. However, the centerpiece of our approach is the application of time-resolved laser transient absorption spectroscopy to this problem. This method allows the direct observation of the photolabeling process and provides detailed information on the key activation and bonding steps. The laser spectroscopic results will be combined with the results of other, more classical, experiments to accomplish the aims of this reasearch.