STUDY OF PHOTOAFFINITY LABELS BY LASER SPECTROSCOPY

通过激光光谱研究光亲和标签

• 批准号: 3275469
• 负责人: GARY B SCHUSTER
• 金额: $ 10.2万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 1980
• 资助国家: 美国
• 起止时间: 1980-07-01 至 1991-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3275469
• 关键词: affinity labeling; azides; chemical group; chemical structure; covalent bond; diazo compound; laser spectrometry; molecular site; photochemistry; photolysis

The major objective of this work is to provide the knowledge necessary for the sound application and interpretation of photolabeling experiments. A major objective in the study of living systems is to associate function with chemical structure. Several experimental approaches have been developed to facilitate this goal. Among the most useful of these are photolabeling procedures. In this approach an activatable reagent is positioned adjacent to a target structure. This is often accomplished by taking advantage of the high affinity of the target for a particular substrate. The key steps in the labeling process involve activation of the latent reactivity of the reagent by irradiation with light and covalent bond formation between the reagent and the target biological structure. Once this has been accomplished, the target structure can be broken into smaller parts and the linked label located. The aim of the research in this application is to examine the details of activation of the photolabeling agent by light, study the process of covalent bond formation, develop new photochemicals useful as labels, and test these findings in actual biological systems. A number of methods will be employed to accomplish these goals. However, the centerpiece of our approach is the application of time-resolved laser transient absorption spectroscopy to this problem. This method allows the direct observation of the photolabeling process and provides detailed information on the key activation and bonding steps. The laser spectroscopic results will be combined with the results of other, more classical, experiments to accomplish the aims of this research.

这项工作的主要目标是提供必要的知识， 光标记实验的合理应用和解释。 一 生命系统研究的一个主要目标是将功能 化学结构。 已经有几种实验方法， 为实现这一目标而开发。 其中最有用的是 光标记程序。 在该方法中， 定位在目标结构附近。 这通常是通过以下方式实现的： 利用靶对特定的 衬底 标记过程中的关键步骤包括激活 通过光和共价键照射试剂的潜在反应性 在试剂和目标生物结构之间形成键。 一旦这已经完成，目标结构可以被分解成 较小的部件和链接的标签。 研究的目的是 此应用程序是检查激活的细节， 光标记剂通过光，研究共价键形成的过程， 开发新的可用作标记的光化学物质，并在 真实的生物系统 将采用多种方法， 实现这些目标。 然而，我们方法的核心是 时间分辨激光瞬态吸收光谱法在 这个问题 这种方法允许直接观察 光标记过程，并提供了详细的信息，关键 活化和结合步骤。 激光光谱的结果将是 结合其他更经典的实验结果， 实现本研究的目的。