PHOTOAFFINITY LABELS BY LASER SPECTROSCOPY

这项工作的主要目标是提供必要的知识 光标记实验的声音应用与解释。一个 生命系统研究的主要目标是联系功能 具有化学结构。已经有几种实验性的方法 为实现这一目标而开发的。其中最有用的是 光标记程序。在这种方法中，可活化试剂是 与目标结构相邻的位置。这通常通过以下方式实现 利用目标对特定目标的高亲和力 底物。贴标签过程中的关键步骤涉及激活 试剂在光照和共价作用下的潜在反应性 试剂与靶生物结构之间形成键。 一旦完成了这一点，就可以将目标结构分解成 找到较小的零件和链接的标签。这项研究的目的是 此应用程序用于检查激活的详细信息 光标记剂，研究共价键的形成过程， 开发可用作标签的新光化学物质，并在 实际的生物系统。将采用多种方法来 完成这些目标。然而，我们方法的核心是 时间分辨激光瞬时吸收光谱分析技术的应用 这个问题。这种方法允许直接观察 光标记过程，并提供了有关密钥的详细信息 激活和粘合步骤。激光光谱结果将是 结合其他更经典的实验的结果， 完成本研究的目的。

The major objective of this work is to provide the knowledge necessary for the sound application and interpretation of photolabeling experiments. A major objective in the study of living systems is to associate function with chemical structure. Several experimental approaches have been developed to facilitate this goal. Among the most useful of these are photolabeling procedures. In this approach an activatable reagent is positioned adjacent to a target structure. This is often accomplished by taking advantage of the high affinity of the target for a particular substrate. The key steps in the labeling process involve activation of the latent reactivity of the reagent by irradiation with light and covalent bond formation between the reagent and the target biological structure. Once this has been accomplished, the target structure can be broken into smaller parts and the linked label located. The aim of the research in this application is to examine the details of activation of the photolabeling agent by light, study the process of covalent bond formation, develop new photochemicals useful as labels, and test these findings in actual biological systems. A number of methods will be employed to accomplish these goals. However, the centerpiece of our approach is the application of time-resolved laser transient absorption spectroscopy to this problem. This method allows the direct observation of the photolabeling process and provides detailed information on the key activation and bonding steps. The laser spectroscopic results will be combined with the results of other, more classical, experiments to accomplish the aims of this research.