STUDY OF PHOTOAFFINITY LABELS BY LASER SPECTROSCOPY

通过激光光谱研究光亲和标签

• 批准号: 3275474
• 负责人: GARY B SCHUSTER
• 金额: $ 11.46万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 1980
• 资助国家: 美国
• 起止时间: 1980-07-01 至 1995-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3275474
• 关键词: adenosine; affinity labeling; azides; chemical group; chemical reaction; chemical structure; covalent bond; crosslink; iodine; laser spectrometry; molecular site; nitrene; nitro compounds; phosphorus compounds; photochemistry; photolysis; pyridine; technology /technique development

The major objective of this work is to provide the knowledge necessary for sound application and interpretation of photolabeling experiments. A major objective in the study of living systems is to associate function with chemical structure. Several experimental approaches have been developed to facilitate this goal. Among the most useful of these are photolabeling procedures. In this approach an activatable reagent is positioned adjacent to a target structure. This is often accomplished by taking advantage of the high affinity of the target for a particular substrate. The key steps in the labeling process involve activation of the latent reactivity of the reagent by irradiation with light and covalent bond formation between the reagent and the target biological structure. Once this has been accomplished, the target structure can be broken into smaller parts and the linked label located. The aim of the research in this application is to examine the details of activation of the photolabeling agent by light, study the process of covalent bond formation, develop new photochemicals useful as labels, and test these findings in actual biological systems. A number of methods will be employed to accomplish these goals. However, the centerpiece of our approach is the application of time-resolved laser transient absorption spectroscopy to this problem. This method allows the direct observation of the photolabeling process and provides detailed information on the key activation and bonding steps. The laser spectroscopic results will be combined with the results of other, more classical, experiments to accomplish the aims of this reasearch.

这项工作的主要目标是提供必要的知识 用于光标记实验的合理应用和解释。一 生命系统研究的一个主要目标是将功能 化学结构。已经有几种实验方法， 为实现这一目标而开发。 其中最有用的是 光标记程序。 在该方法中， 定位在目标结构附近。 这往往是实现 通过利用目标对特定分子的高亲和力， 衬底 标记过程中的关键步骤包括激活 试剂通过光照射的潜在反应性， 试剂和目标生物之间的共价键形成 结构 一旦完成了这一点，目标结构可以是 分解成更小的部分并定位链接的标签。 的目的 本申请中的研究是检查 光标记剂通过光的照射，研究共价键的形成过程 形成，开发新的光化学品作为标签，并测试这些 在实际生物系统中的发现。 一些方法将 来实现这些目标。 然而，我们的核心 方法是应用时间分辨激光瞬态吸收 spectroscopy光谱to this problem问题. 这种方法允许直接观察 的光标记过程，并提供了详细的信息， 键激活和键合步骤。 激光光谱结果将 与其他更经典的实验结果相结合， 实现这一复兴的目标。