IDENTIFICATION OF FACTORS CONFERRING CLOCK REGULATION

确定影响时钟调节的因素

• 批准号: 2242537
• 负责人: HYESEON CHO
• 金额: $ 2.37万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-03-15 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2242537
• 关键词: IDENTIFICATION; FACTORS; CONFERRING; CLOCK; REGULATION

The cellular machinery that generates endogenous temporal control and organization known collectively as the biological clock, exists in virtually all eukaryotic organisms. The clock influences many aspects of human physiology including work-rest cycles, and drug tolerance band effectiveness, and its malfunction is known to be associated with common human disorders such as insomnia and manic-depressive illness. Extensive research in the past has demonstrated the extent and significance of clock control on cellular and organismal processes; however, the cellular and molecular basis of the biological clock and its control mechanisms are largely unknown. Therefore, the long-term goal of the study of the clock and clock regulation is to define the genetic and biochemical components of the clock, and understand how these components interact to keep functional time. This study will use molecular genetics to identify cis-acting elements and trans-acting factors conferring clock regulation using a simple eukaryotic model system, Neurospora crassa. First, minimal cis-acting DNA elements including a circadian clock-responsive element and a light- responsive element will be determined by deletion analysis of the 5' upstream regulatory region of the clock-controlled gene-1 (ccg-1). The minimal CCRE driving a selectable reporter such as hygromycin phosphotransferase will be then used in a genetic screen to identify the gene(s) encoding the trans-acting factors conferring morning-specific clock regulation. Characterization and functional studies of the identified factor(s) will lead to an understanding of the mechanism by which the clock exerts its control on the clock-controlled processes.

产生内源性时间控制和 统称为生物钟的组织存在于 几乎所有的真核生物。时钟影响着许多方面 人体生理学，包括作息周期和药物耐受区 有效性，而它的故障已知与常见的 人类疾病，如失眠和躁郁症。广泛性 过去的研究已经证明了 细胞和生物体过程的时钟控制；然而，细胞 生物钟的分子基础及其调控机制 在很大程度上是未知的。因此，研究的长期目标是 生物钟的调节是定义遗传和生化的 时钟的组件，并了解这些组件如何相互作用 保持正常工作时间。 这项研究将使用分子遗传学来鉴定顺式作用元件。 和授权时钟调节的事务因素使用简单 真核模型系统，粗糙脉孢菌。首先，最低限度的顺式作用DNA 包括昼夜时钟响应元件和光- 响应元件将通过5‘端缺失分析来确定 时钟控制基因-1(CCG-1)的上游调控区。这个 最小CCRE驱动可选择的报告，如潮霉素 然后将在基因筛查中使用磷酸转移酶来识别 编码清晨特异性反式作用因子的基因(S) 时钟调节。血管内皮细胞的特性和功能研究 确定的因素(S)将导致对机制的理解 时钟对时钟控制的进程施加其控制。