Tuning Molecular Translocaton by Close-Field Electroporation

通过近场电穿孔调节分子易位

• 批准号: DP150104754
• 负责人: Prof Gary Housley
• 金额: $ 26.54万
• 依托单位: The University of New South Wales
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2015
• 资助国家: 澳大利亚
• 起止时间: 2015-02-10 至 2018-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP150104754
• 关键词: Tuning; Molecular; Translocaton; Close; Field

This project aims to determine the underlying mechanisms by which DNA and other molecules are able to migrate across the cell membrane in response to highly localised electric fields. It has recently been shown that focusing of electric fields at the cellular level, using an array of small electrodes, results in unexpectedly high cell transfection efficiencies. It has been termed 'close-field electroporation'. Here it is proposed to establish the properties of the electric fields around cells and cell membrane interactions with these fields that enable molecular translocation. This fundamental science could have broad implications in the domains of drug delivery, gene therapy and neural stimulation.

该项目旨在确定DNA和其他分子能够响应高度局部化的电场而迁移穿过细胞膜的潜在机制。 最近的研究表明，使用小电极阵列将电场聚焦在细胞水平，会导致意想不到的高细胞转染效率。 它被称为“近场电穿孔”。 在这里，它建议建立周围的细胞和细胞膜与这些领域的相互作用，使分子易位的电场的属性。 这一基础科学可能在药物输送、基因治疗和神经刺激等领域产生广泛的影响。