AFFERENT MODULATION OF RESPIRATORY PATTERN IN VITRO

将使用分离的新生大鼠脑干/脊髓准备 为了研究神经种群之间的相互作用 呼吸节律的产生和重置这一点的传入输入 节奏。在这个准备过程中，产生呼吸节律的网络是 全功能，它们的细胞和突触特性很容易 无障碍。内源性猝发细胞(其膜电位为 在没有突触输入的情况下振荡)已经在 准备工作。在某些呼吸节律发生的模型中，内源性 爆发者通过他们的爆发频率来确定呼吸频率， 这是已知的它们静息膜电位的函数。 初步研究表明，呼吸节律是由电信号重新设定的 这种制剂对迷走神经有刺激作用。的影响 刺激是在呼吸周期中发生的时间的函数： 当在灵感过程中施加刺激时，灵感会被缩短， 当早期实施刺激措施时，有效期会延长 到期，并在下半年应用刺激措施时缩短 过期了。如果内源性猝发决定了呼吸频率，那么 刺激诱导的呼吸节律重置必须收敛于这些 细胞。在刺激诱导重置期间监测内源性猝发 呼吸节律将检验这一假设，并区分 在呼吸功能回路的不同模型之间 节律发生。 细胞和突触机制的特征 中央产生的节律和传入反馈的相互作用可能会消失 浅谈呼吸道疾病的神经基础 中央产生的节律和感官的不适当耦合 反馈。在这种准备中，这种病理的神经基础可以 正在进行调查，并开发新的治疗干预措施。

The isolated neonate rat brainstem/spinal cord preparation will be used to investigate the interaction between neural populations mediating the generation of respiratory rhythm and afferent inputs that reset this rhythm. In this preparation, networks generating respiratory rhythm are fully functional, and their cellular and synaptic properties are readily accessible. Endogenous bursters (cells whose membrane potentials oscillate in the absence of synaptic input) have been identified in this preparation. In some models of respiratory rhythmogenesis, endogenous bursters determine respiratory frequency by their bursting frequency, which is known to be a function of their resting membrane potentials. Preliminary studies show that respiratory rhythm is reset by electrical stimulation to the vagus nerve in this preparation. The effect of stimulation is a function of when it occurs in the respiratory cycle: inspiration is shortened when the stimulus is applied during inspiration, expiration is lengthened when the stimulus is applied early in expiration, and shortened when the stimulus is applied late in expiration. If endogenous bursters determine respiratory frequency, then stimulus-induced resetting of respiratory rhythm must converge on these cells. Monitoring endogenous bursters during stimulus-induced resetting of respiratory rhythm will test this hypothesis, and differentiate between alternative models of the functional circuitry for respiratory rhythmogenesis. Characterizing the cellular and synaptic mechanisms mediating the interaction of centrally generated rhythm and afferent feedback may shed light on the neural basis of respiratory pathologies which arise out of the inappropriate coupling of centrally generated rhythm and sensory feedback. In this preparation the neural basis for such pathologies can be investigated, and novel therapeutic interventions developed.