NUTRIENT TRANSPORT TO MOSQUITO OOCYTES

所有的昆虫都为它们的卵提供必需的营养。 未能提供 这些营养素会导致胚胎发育失败。 确定运输和隔离的分子事件 鸡蛋中的营养物质应该为特定控制提供新的目标， 害虫，尤其是人类和动物疾病的媒介。 我们将研究两种必需营养素的运输--铁和 脂质--埃及伊蚊的卵母细胞。由于大人 为了产卵，雌性必须以脊椎动物的血液为食，我们将 研究血粉中无机铁和血红素铁的利用 用于鸡蛋生产。 重点将放在运输载体蛋白上 通过血淋巴和卵母细胞的吸收和储存。 这将 涉及铁结合蛋白、转铁蛋白和铁蛋白以及受体 让铁进入细胞 脂质从脂肪体到卵母细胞的运输可能涉及释放， 通过脂质转运颗粒促进，通过脂蛋白转运， 脂蛋白脂酶和受体促进卵巢卸载 将脂蛋白结合到细胞膜上。将试图 评估干预各种功能的有效性 大分子成分 最终目标是提供 将允许开发抑制昆虫的特定材料 生殖

All insects provide essential nutrients to their eggs. Failure to provide these nutrients will result in failure of the embryo to develop. Determination of the molecular events of transport and sequestration of nutrients in the egg should provide new targets for specific control of insect pests, especially vectors of human and animal diseases. We will investigate the transport of two essential nutrients--iron and lipids--to the oocytes of the mosquito Aedes aegypti. Since the adult female must feed on vertebrate blood in order to produce eggs, we will study the utilization of both inorganic and heme iron from the blood meal for egg production. Focus will be on the carrier proteins for transport through the hemolymph and the uptake and storage in the oocyte. This will involve iron-binding proteins, transferrin and ferritin, and receptors that allow iron to enter cells. Lipid transport from the fat body to the oocyte involves release, probably facilitated by a lipid transport particle, transport by lipoproteins, unloading at the ovary facilitated by lipoprotein lipase and a receptor for binding the lipoprotein to the cell membrane. Attempts will be made to assess the effectiveness of interfering with the function of the various macromolecular components. The ultimate goal is to provide systems that will allow development of specific materials that inhibit insect reproduction.